Department of Immunology, IIS-Fundación Jimenez Diaz, Madrid, Spain.
Center for Plant Biotechnology and Genomic (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain.
Allergy. 2015 Aug;70(8):1024-7. doi: 10.1111/all.12646. Epub 2015 May 27.
Food allergy is recognized as a major public health issue, especially in early childhood. It has been hypothesized that early sensitization to food allergens maybe due to their ingestion as components dissolved in the milk during the breastfeeding, explaining reaction to a food, which has never been taken before. Thus, the aim of this work has been to detect the presence of the food allergens in breast milk by microarray technology. We produced a homemade microarray with antibodies produced against major food allergens. The antibody microarray was incubated with breast milk from 14 women collected from Fundación Jiménez Díaz Hospital. In this way, we demonstrated the presence of major foods allergens in breast milk. The analysis of allergens presented in breast milk could be a useful tool in allergy prevention and could provide us a key data on the role of this feeding in tolerance induction or sensitization in children.
食物过敏被认为是一个主要的公共卫生问题，尤其是在儿童早期。有人假设，早期对食物过敏原的致敏可能是由于它们作为成分溶解在母乳中被摄入，从而解释了对从未吃过的食物的反应。因此，本工作的目的是通过微阵列技术检测母乳中食物过敏原的存在。我们用针对主要食物过敏原的抗体生产了一种自制的微阵列。将抗体微阵列与来自 14 名妇女的母乳在 Fundación Jiménez Díaz 医院收集。通过这种方式，我们证明了母乳中存在主要食物过敏原。分析母乳中存在的过敏原可能是预防过敏的有用工具，并为我们提供关于这种喂养在诱导儿童耐受或致敏方面的作用的关键数据。
