用固定化生长因子或肽对基于胶原蛋白的生物材料进行修饰。
Modifications of collagen-based biomaterials with immobilized growth factors or peptides.
作者信息
Xiao Yun, Reis Lewis A, Zhao Yimu, Radisic Milica
机构信息
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
出版信息
Methods. 2015 Aug;84:44-52. doi: 10.1016/j.ymeth.2015.04.025. Epub 2015 May 4.
In order to provide an instructive microenvironment to facilitate cellular behaviors and tissue regeneration, biomaterials can be modified by immobilizing growth factors or peptides. We describe here our procedure for modification of collagen-based biomaterials, both porous scaffolds and hydrogel systems, with growth factors or peptides by covalent immobilization. Characterizations of the modified biomaterials (immobilization efficiency, release profile, morphology, mechanical strength, and rheology) and in vitro testing with cells are also discussed.
为了提供一个具有指导意义的微环境以促进细胞行为和组织再生，可以通过固定生长因子或肽来修饰生物材料。我们在此描述通过共价固定用生长因子或肽修饰基于胶原蛋白的生物材料（多孔支架和水凝胶系统）的过程。还讨论了修饰后生物材料的表征（固定效率、释放曲线、形态、机械强度和流变学）以及细胞的体外测试。
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