Aryaei Ashkan, Jayasuriya Ambalangodage C
Department of Mechanical Engineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA.
Department of Orthopaedic Surgery, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USA.
Mater Sci Eng C Mater Biol Appl. 2015;52:129-34. doi: 10.1016/j.msec.2015.03.024. Epub 2015 Mar 21.
The aim of this paper is to investigate the effect of the magnitude and duration of oscillatory mechanical stimulation on osteoblast attachment and proliferation as well as the time gap between seeding and applying the stimulation. Cells were exposed to three levels of speed at two different conditions. For the first group, mechanical shear stress was applied after 20 min of cell seeding. For the second group there was no time gap between cell seeding and applying mechanical stimulation. The total area subjected to shear stress was divided into three parts and for each part a comparative study was conducted at defined time points. Our results showed that both shear stress magnitude and the time gap between cell seeding and applying shear stress, are important in further cell proliferation and attachment. The effect of shear stress was not significant at lower speeds for both groups at earlier time points. However, a higher percentage of area was covered by cells at later time points under shear stress. In addition, the time gap can also improve osteoblast attachment. For the best rate of cell attachment and proliferation, the magnitude of shear stress and time gap should be optimized. The results of this paper can be utilized to improve cell attachment and proliferation in bioreactors.
本文旨在研究振荡机械刺激的强度和持续时间对成骨细胞附着和增殖的影响，以及接种细胞与施加刺激之间的时间间隔。在两种不同条件下，使细胞暴露于三种速度水平。对于第一组，在细胞接种20分钟后施加机械剪切应力。对于第二组，在细胞接种和施加机械刺激之间没有时间间隔。将承受剪切应力的总面积分为三个部分，并在规定的时间点对每个部分进行比较研究。我们的结果表明，剪切应力大小以及细胞接种与施加剪切应力之间的时间间隔，对于细胞的进一步增殖和附着都很重要。在早期时间点，两组在较低速度下剪切应力的影响均不显著。然而，在后期时间点，承受剪切应力的区域细胞覆盖百分比更高。此外，时间间隔也可以改善成骨细胞的附着。为了实现最佳的细胞附着和增殖速率，应优化剪切应力的大小和时间间隔。本文的结果可用于改善生物反应器中的细胞附着和增殖。
