Wu Xiaoli, Meng Guolong, Wang Shanling, Wu Fang, Huang Wanxia, Gu Zhongwei
College of Materials Science and Engineering, Sichuan University, Chengdu, PR China.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, PR China.
Mater Sci Eng C Mater Biol Appl. 2015;52:242-50. doi: 10.1016/j.msec.2015.03.057. Epub 2015 Mar 26.
Essential element like Zn or Sr is known to play an important role in bone remodeling process. In this study, we have used the sol-gel process to synthesize the Zn (2%) and Sr (5%) doped 64S bioglasses (BGs, 64SiO2-5P2O5-31CaO, mol.%), alone and co-doped. The synthesized glasses were characterized by XRD, FTIR and STEM. For biological evaluation, the effects of Zn and Sr incorporation on the in vitro bioactivity of the synthesized BGs were studied using the simulated body fluid (SBF) soaking. The proliferation and differentiation (ALP, OCN) of rat mesenchymal stem cells (MSCs) on these BGs were studied using CCK-8 and ELISA analyses. The results indicated that Zn had been uniformly incorporated into the bioglass, and demonstrated a stimulating effect on apatite-like layer formation, MSC proliferation and differentiation. On the other hand, most of Sr appeared to form a secondary crystal phase with extremely high solubility in SBF, showing an enhancing effect only in MSC differentiation but not in proliferation, as well as an inhibitory effect on apatite-like layer formation. The different dissolution behaviors of Sr and Zn ions seemed to have a strong correlation with the different apatite-like layer formation capabilities and the cellular responses of Zn and Sr containing BGs.
已知锌或锶等必需元素在骨重塑过程中发挥重要作用。在本研究中，我们采用溶胶 - 凝胶法合成了单独掺杂锌（2%）和锶（5%）以及共掺杂的64S生物玻璃（BGs，64SiO₂ - 5P₂O₅ - 31CaO，摩尔百分比）。通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描透射电子显微镜（STEM）对合成的玻璃进行了表征。为了进行生物学评估，使用模拟体液（SBF）浸泡研究了锌和锶的掺入对合成的BGs体外生物活性的影响。使用CCK - 8和酶联免疫吸附测定（ELISA）分析研究了大鼠间充质干细胞（MSCs）在这些BGs上的增殖和分化（碱性磷酸酶，骨钙素）。结果表明，锌已均匀掺入生物玻璃中，并对类磷灰石层的形成、MSC增殖和分化具有刺激作用。另一方面，大部分锶似乎形成了在SBF中具有极高溶解度的二次晶相，仅对MSC分化有增强作用，对增殖无增强作用，并且对类磷灰石层的形成有抑制作用。锶和锌离子不同的溶解行为似乎与含锌和含锶BGs不同的类磷灰石层形成能力以及细胞反应密切相关。
