Gavinho Sílvia R, Pádua Ana Sofia, Sá-Nogueira Isabel, Silva Jorge C, Borges João P, Costa Luis C, Graça Manuel Pedro F
I3N and Physics Department, Aveiro University, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
I3N-CENIMAT and Physics Department, NOVA School of Science and Technology, Campus de Caparica, 2829-516 Caparica, Portugal.
Materials (Basel). 2023 Jan 19;16(3):956. doi: 10.3390/ma16030956.
Polymeric membranes are widely used in guided bone regeneration (GBR), particularly in dentistry. In addition, bioactive glasses can be added to the polymers in order to develop a matrix that is osteoconductive and osteoinductive, increasing cell adhesion and proliferation. The bioactive glasses allow the insertion into its network of therapeutic ions in order to add specific biological properties. The addition of zinc into bioactive glasses can promote antibacterial activity and induce the differentiation and proliferation of the bone cells. In this study, bioactive glasses containing zinc (0.25, 0.5, 1 and 2 mol%) were developed and structurally and biologically characterized. The biological results show that the Zn-containing bioactive glasses do not present significant antibacterial activity, but the addition of zinc at the highest concentration does not compromise the bioactivity and promotes the viability of Saos-2 cells. The cell culture assays in the membranes (PCL, PCL:BG and PCL:BGZn2) showed that zinc addition promotes cell viability and an increase in alkaline phosphatase (ALP) production.
聚合物膜广泛应用于引导骨再生（GBR），尤其是在牙科领域。此外，可以将生物活性玻璃添加到聚合物中，以开发一种具有骨传导性和骨诱导性的基质，增加细胞黏附和增殖。生物活性玻璃允许治疗性离子插入其网络中，以赋予特定的生物学特性。向生物活性玻璃中添加锌可以促进抗菌活性，并诱导骨细胞的分化和增殖。在本研究中，制备了含锌（0.25、0.5、1和2 mol%）的生物活性玻璃，并对其结构和生物学特性进行了表征。生物学结果表明，含锌生物活性玻璃没有显著的抗菌活性，但添加最高浓度的锌不会损害生物活性，反而能促进Saos-2细胞的活力。在膜（PCL、PCL:BG和PCL:BGZn2）中进行的细胞培养试验表明，添加锌可促进细胞活力并增加碱性磷酸酶（ALP）的产生。
