高脂饮食诱导的发情周期紊乱：粗叶榕的影响。

High fat diet-induced estrus cycle disruption: effects of Ficus asperifolia.

作者信息

Ngadjui Esther, Nkeng-Efouet Pepin Alango, Nguelefack Telesphore Benoit, Kamanyi Albert, Watcho Pierre

出版信息

J Complement Integr Med. 2015 Sep;12(3):205-15. doi: 10.1515/jcim-2014-0074.

DOI:10.1515/jcim-2014-0074

PMID:25955283

Abstract

BACKGROUND

Ficus asperifolia (L) Hook. Ex Miq (Moraceae) fruits are used in Cameroonian traditional medicine to cure some cases of infertility in women. This study determines the mechanism of alleviating effect of the plant extracts on rat infertility induced by a high fat diet (HFD).

METHODS

Obesity was reached by feeding female rats with a HFD for 10 weeks. Vaginal smear was observed daily for 3 weeks after animals were obese. Then, 70 animals with abnormal estrus cyclicity were selected and partitioned into two sets of 35 animals. Each set was further divided into seven groups of five rats. These obese rats with disrupted estrus cyclicity were orally administered the aqueous and methanolic extracts (100 and 500 mg/kg), distilled water (10 mL/kg), 5% Tween 80 (10 mL/kg) or lutenyl (0.8 µg/kg) once a day for 1 week (set I) or 4 weeks (set II). Estrus cyclicity, body weight gain, hematocrit, lipid profile, ovarian, uterine and hepatic growth indices were determined at the end of each treatment.

RESULTS

HFD increased the body weight of the animals by 27% and disrupted the estrus cyclicity by 98.44%. Aqueous extract (100 mg/kg) of F. asperifolia given for 1 week corrected 40% of the irregular estrus cycle and this percentage increased to 80% as the treatment progressed to 4 weeks. F. asperifolia-treated obese rats (mostly with the aqueous extract at 100 mg/kg) showed a significant decrease (p<0.001) in the total plasma cholesterol and low density lipoprotein (LDL) cholesterol level and a significant increase (p<0.001) in high density lipoprotein (HDL) cholesterol. F. asperifolia has bioactive agents that may maintain conducive conditions for reproduction in obese female rats.

CONCLUSIONS

Our data support the anecdotal claims of F. asperifolia in folk medicine to cure some cases of infertility in women.

摘要

背景

粗糙榕（桑科）果实被用于喀麦隆传统医学中治疗某些女性不孕症病例。本研究确定了该植物提取物对高脂饮食（HFD）诱导的大鼠不孕症的缓解作用机制。

方法

通过给雌性大鼠喂食HFD 10周来诱导肥胖。动物肥胖后，每天观察阴道涂片3周。然后，选择70只发情周期异常的动物，分成两组，每组35只。每组再进一步分为七组，每组五只大鼠。给这些发情周期紊乱的肥胖大鼠每天口服水提取物和甲醇提取物（100和500毫克/千克）、蒸馏水（10毫升/千克）、5%吐温80（10毫升/千克）或氯米芬（0.8微克/千克），持续1周（第一组）或4周（第二组）。在每次治疗结束时测定发情周期、体重增加、血细胞比容、血脂谱、卵巢、子宫和肝脏生长指数。

结果

HFD使动物体重增加了27%，并使发情周期紊乱率达到98.44%。给予1周的粗糙榕水提取物（100毫克/千克）纠正了40%的不规则发情周期，随着治疗进展到4周，这一百分比增加到80%。经粗糙榕治疗的肥胖大鼠（大多使用100毫克/千克的水提取物）血浆总胆固醇和低密度脂蛋白（LDL）胆固醇水平显著降低（p<0.001），高密度脂蛋白（HDL）胆固醇显著升高（p<0.001）。粗糙榕含有生物活性剂，可能为肥胖雌性大鼠维持有利于繁殖的条件。