Cianciolo R E, Mohr F C, Aresu L, Brown C A, James C, Jansen J H, Spangler W L, van der Lugt J J, Kass P H, Brovida C, Cowgill L D, Heiene R, Polzin D J, Syme H, Vaden S L, van Dongen A M, Lees G E
Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA
Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA.
Vet Pathol. 2016 Jan;53(1):113-35. doi: 10.1177/0300985815579996. Epub 2015 May 8.
Evaluation of canine renal biopsy tissue has generally relied on light microscopic (LM) evaluation of hematoxylin and eosin-stained sections ranging in thickness from 3 to 5 µm. Advanced modalities, such as transmission electron microscopy (TEM) and immunofluorescence (IF), have been used sporadically or retrospectively. Diagnostic algorithms of glomerular diseases have been extrapolated from the World Health Organization classification scheme for human glomerular disease. With the recent establishment of 2 veterinary nephropathology services that evaluate 3-µm sections with a panel of histochemical stains and routinely perform TEM and IF, a standardized objective species-specific approach for the diagnosis of canine glomerular disease was needed. Eight veterinary pathologists evaluated 114 parameters (lesions) in renal biopsy specimens from 89 dogs. Hierarchical cluster analysis of the data revealed 2 large categories of glomerular disease based on the presence or absence of immune complex deposition: The immune complex-mediated glomerulonephritis (ICGN) category included cases with histologic lesions of membranoproliferative or membranous patterns. The second category included control dogs and dogs with non-ICGN (glomerular amyloidosis or focal segmental glomerulosclerosis). Cluster analysis performed on only the LM parameters led to misdiagnosis of 22 of the 89 cases-that is, ICGN cases moved to the non-ICGN branch of the dendrogram or vice versa, thereby emphasizing the importance of advanced diagnostic modalities in the evaluation of canine glomerular disease. Salient LM, TEM, and IF features for each pattern of disease were identified, and a preliminary investigation of related clinicopathologic data was performed.
犬肾活检组织的评估通常依赖于对苏木精和伊红染色切片进行光镜（LM）评估，切片厚度为3至5微米。诸如透射电子显微镜（TEM）和免疫荧光（IF）等先进技术只是偶尔或回顾性地使用。肾小球疾病的诊断算法是从世界卫生组织人类肾小球疾病分类方案中推断出来的。随着最近建立了2个兽医肾脏病学服务机构，它们使用一组组织化学染色评估3微米切片，并常规进行TEM和IF，因此需要一种标准化的、客观的、针对特定物种的方法来诊断犬肾小球疾病。8名兽医病理学家评估了89只犬肾活检标本中的114个参数（病变）。对数据进行层次聚类分析后发现，根据免疫复合物沉积的有无，肾小球疾病可分为两大类：免疫复合物介导的肾小球肾炎（ICGN）类别包括具有膜增生性或膜性组织学病变的病例。第二类包括对照犬和患有非ICGN（肾小球淀粉样变性或局灶节段性肾小球硬化症）的犬。仅对LM参数进行聚类分析导致89例病例中有22例误诊，即ICGN病例移至树状图的非ICGN分支，反之亦然，从而强调了先进诊断技术在评估犬肾小球疾病中的重要性。确定了每种疾病模式的显著LM、TEM和IF特征，并对相关临床病理数据进行了初步调查。
