Zong Jianfa, Wang Ruilong, Bao Guanhu, Ling Tiejun, Zhang Liang, Zhang Xinfu, Hou Ruyan
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China.
Fitoterapia. 2015 Jul;104:7-13. doi: 10.1016/j.fitote.2015.05.001. Epub 2015 May 7.
Four oleanane-type triterpenoid saponins were isolated from the seed cake of Camellia oleifera Abel.: camelliasaponin B1 and three new saponins, oleiferasaponin C1-C3 (1-3). Their structures were identified as 22-O-angeloyl-camelliagenin B 3-O-[β-d-galactopyranosyl-(1→2)]-[β-d-galactopyranosyl-(1→2)-α-l-arabinopyranosyl-(1→3)]-β-d-glucopyranosiduronic acid methyl ester (1); 22-O-angeloyl-camelliagenin A 3-O-[β-d-galactopyranosyl-(1→2)]-[β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→3)]-β-d-glucopyranosiduronic acid methyl ester (2); and 28-O-cinnamoyl-camelliagenin B 3-O-[β-d-galactopyranosylz-(1→2)] [β-d-galactopyranosyl(1→2)-α-l-arabinopyranosyl-(1→3)]-β-d-glucopyranosiduronic acid methyl ester (3) through 1D and 2D NMR, HR-ESI-MS, as well as GC-MS spectroscopic methods. The anti-proliferative activities of these four compounds were investigated on five human tumor cell lines (BEL-7402, BGC-823, MCF-7, HL-60 and KB). Compounds 1 and 2 and camelliasaponin B1 showed significant cytotoxic activities.
茶皂苷B1和三种新皂苷，油茶皂苷C1 - C3（1 - 3）。通过一维和二维核磁共振、高分辨电喷雾电离质谱以及气相色谱 - 质谱光谱方法，将它们的结构鉴定为22 - O - 当归酰基 - 茶皂苷元B 3 - O - [β - D - 吡喃半乳糖基 - (1→2)] - [β - D - 吡喃半乳糖基 - (1→2) - α - L - 阿拉伯吡喃糖基 - (1→3)] - β - D - 吡喃葡萄糖醛酸甲酯（1）；22 - O - 当归酰基 - 茶皂苷元A 3 - O - [β - D - 吡喃半乳糖基 - (1→2)] - [β - D - 吡喃葡萄糖基 - (1→2) - β - D - 吡喃半乳糖基 - (1→3)] - β - D - 吡喃葡萄糖醛酸甲酯（2）；以及28 - O - 肉桂酰基 - 茶皂苷元B 3 - O - [β - D - 吡喃半乳糖基 - (1→2)] [β - D - 吡喃半乳糖基(1→2) - α - L - 阿拉伯吡喃糖基 - (1→3)] - β - D - 吡喃葡萄糖醛酸甲酯（3）。研究了这四种化合物对五种人类肿瘤细胞系（BEL - 7402、BGC - 823、MCF - 7、HL - 60和KB）的抗增殖活性。化合物1、2和茶皂苷B1表现出显著的细胞毒活性。
