Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam 42300, Selangor Darul Ehsan, Malaysia.
Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam 42300, Selangor Darul Ehsan, Malaysia.
Nutrients. 2020 Sep 30;12(10):2993. doi: 10.3390/nu12102993.
is a miracle plant with many nutritional and medicinal properties. Chemopreventive values of the combined mixture of moringa leaves and seed residue (MOLSr) at different ratios (M1S9, M1S1 and M9S1) were investigated. MOLSr extracts were subjected to phytochemical screening, antioxidant assays, metabolite profiling and cytotoxic activity on the primary mammary epithelial cells (PMECs), non-malignant Chang's liver cells and various human cancer cell lines (including breast, cervical, colon and liver cancer cell lines). The MOLSr ratio with the most potent cytotoxic activity was used in xenograft mice injected with MDA-MB-231 cells for in vivo tumorigenicity study as well as further protein and gene expression studies. M1S9, specifically composed of saponin and amino acid, retained the lowest antioxidant activity but the highest glucosinolate content as compared to other ratios. Cell viability decreased significantly in MCF-7 breast cancer cells and PMECs after treatment with M1S9. Solid tumor from MDA-MB-231 xenograft mice was inhibited by up to 64.5% at third week after treatment with high-dose M1S9. High-dose M1S9 significantly decreased the expression of calcineurin (CaN) and vascular endothelial cell growth factor (VEGF) proteins as well as the secreted frizzled-related protein 1 (SFRP1) and solute carrier family 39 member 6 (SLC39A6) genes. This study provides new scientific evidence for the chemoprevention potential of MOLSr extracts in a breast cancer model; however, the precise mechanism warrants further investigation.
辣木是一种具有多种营养和药用特性的神奇植物。研究了辣木叶和种子残渣（MOLSr）不同比例（M1S9、M1S1 和 M9S1）混合物的化学预防价值。对 MOLSr 提取物进行了植物化学筛选、抗氧化剂测定、代谢物分析和对原代乳腺上皮细胞（PMECs）、非恶性 Chang 肝细胞和各种人癌细胞系（包括乳腺癌、宫颈癌、结肠癌和肝癌细胞系）的细胞毒性活性分析。在注射 MDA-MB-231 细胞的异种移植小鼠中使用具有最强细胞毒性活性的 MOLSr 比例进行体内致瘤性研究以及进一步的蛋白质和基因表达研究。与其他比例相比，M1S9 特别由皂苷和氨基酸组成，保留了最低的抗氧化活性，但具有最高的硫代葡萄糖苷含量。与对照相比，在用 M1S9 处理后 MCF-7 乳腺癌细胞和 PMECs 的细胞活力显著降低。用高剂量 M1S9 处理后第三周，MDA-MB-231 异种移植小鼠的实体瘤抑制率高达 64.5%。高剂量 M1S9 显著降低了钙调神经磷酸酶（CaN）和血管内皮生长因子（VEGF）蛋白以及分泌卷曲相关蛋白 1（SFRP1）和溶质载体家族 39 成员 6（SLC39A6）基因的表达。这项研究为 MOLSr 提取物在乳腺癌模型中的化学预防潜力提供了新的科学证据；然而，确切的机制需要进一步研究。
