Qiu Yuanxin, He Di, Yang Jingxian, Ma Lukai, Zhu Kaiqi, Cao Yong
School of Light Industry and Food, Zhongkai University of Agricultural and Engineering, Guangzhou, 510220 China.
School of Food Science and Engineering, South China Agricultural University, No. 483 Wushan Road, Wushan Street, Tianhe District, Guangzhou, 510000 China.
Eur Food Res Technol. 2020;246(12):2383-2397. doi: 10.1007/s00217-020-03582-0. Epub 2020 Aug 9.
Natural biologically active substances have received continuous attention for the potentially beneficial health properties against chronic diseases. In this study, bacteriostatic active substance from meal, which is a major by-product of the oil processing industry, were extracted with continuous phase change extraction (CPCE) method and separated by HSCCC. Compared with traditional extraction methods, CPCE possessed higher extraction efficiency. Two main substances were separated and purified (above 90.0%). The structure of them were further identified by UV, LC-ESI-MS-MS, 1H-NMR, and 13C-NMR as flavonoids F2 kaempferol 3--[β-d-glucopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside and J2 kaempferol 3--[β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside for the first time in meal. The results of antibacterial activity measurement showed that both compounds have excellent antibacterial activity. And the antibacterial stability of F2 were finally confirmed: F2 showed broad spectrum antibacterial activity against , , , , and . Besides, F2 exhibited relatively high stable property even at high temperature, acid and metal ion solutions. The findings of this work suggest the possibility of employing meal as an attractive source of health-promoting compounds, and at the same time facilitate its high-value reuse and reduction of environmental burden.
天然生物活性物质因其对慢性疾病潜在的有益健康特性而受到持续关注。在本研究中，采用连续相变化萃取（CPCE）法从油加工行业的主要副产品粕中提取抑菌活性物质，并通过高速逆流色谱（HSCCC）进行分离。与传统萃取方法相比，CPCE具有更高的萃取效率。分离并纯化出两种主要物质（纯度高于90.0%）。通过紫外光谱（UV）、液相色谱-电喷雾电离串联质谱（LC-ESI-MS-MS）、核磁共振氢谱（1H-NMR）和核磁共振碳谱（13C-NMR）首次在粕中鉴定出它们的结构分别为黄酮类化合物F2山奈酚3 - [β-D-吡喃葡萄糖基-(1→2)-α-L-鼠李糖基-(1→6)]-β-D-吡喃葡萄糖苷和J2山奈酚3 - [β-D-吡喃木糖基-(1→2)-α-L-鼠李糖基-(1→6)]-β-D-吡喃葡萄糖苷。抗菌活性测定结果表明这两种化合物均具有优异的抗菌活性。最终证实了F2的抗菌稳定性：F2对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、白色念珠菌、黑曲霉和青霉表现出广谱抗菌活性。此外，F2即使在高温、酸性和金属离子溶液中也表现出相对较高 的稳定性。这项工作的研究结果表明，粕有潜力作为促进健康化合物的诱人来源，同时有助于其高价值再利用并减轻环境负担。
