Peng Yuhong, Yang Yang, Liu Yongkang, Nie Yuanyang, Xu Peilun, Xia Baixue, Tian Fuzhou, Sun Qun
Key Laboratory of Bio-resource and Bio-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China.
The 452nd Hospital of PLA, Chengdu, Sichuan 610000, PR China.
Microb Pathog. 2015 Jun-Jul;83-84:57-63. doi: 10.1016/j.micpath.2015.05.002. Epub 2015 May 7.
The prevalence of cholesterol gallstones has increased in recent years. Bacterial infection correlates with the formation of gallstones. We studied the composition and function of bacterial communities in cholesterol gallstones and bile from 22 cholesterol gallstone patients using culture-dependent and culture-independent methods. Altogether fourteen and eight bacterial genera were detected in cholesterol gallstones and bile, respectively. Pseudomonas spp. were the dominant bacteria in both cholesterol gallstones and bile. As judged by diversity indices, hierarchical clustering and principal component analysis, the bacterial communities in gallstones were different from those in bile. The gallstone microbiome was considered more stable than that of bile. The different microbial communities may be partially explained by differences in their habitats. We found that 30% of the culturable strains from cholesterol gallstones secreted β-glucuronidase and phospholipase A2. Pseudomonas aeruginosa strains showed the highest β-glucuronidase activity and produced the highest concentration of phospholipase A2, indicating that Ps. aeruginosa may be a major agent in the formation of cholesterol gallstones.
近年来，胆固醇结石的患病率有所上升。细菌感染与胆结石的形成相关。我们采用依赖培养和不依赖培养的方法，研究了22例胆固醇结石患者的胆固醇结石及胆汁中细菌群落的组成和功能。在胆固醇结石和胆汁中分别检测到总共14个和8个细菌属。假单胞菌属是胆固醇结石和胆汁中的优势菌。通过多样性指数、层次聚类和主成分分析判断，结石中的细菌群落与胆汁中的不同。胆结石微生物群被认为比胆汁微生物群更稳定。不同的微生物群落可能部分是由其栖息地的差异所解释。我们发现，来自胆固醇结石的可培养菌株中有30%分泌β-葡萄糖醛酸酶和磷脂酶A2。铜绿假单胞菌菌株显示出最高的β-葡萄糖醛酸酶活性，并产生最高浓度的磷脂酶A2，表明铜绿假单胞菌可能是胆固醇结石形成的主要因素。
