Azimirad Masoumeh, Sadeghi Amir, Hosseinkhan Nazanin, Mirbagheri Seyedeh Zohre, Alebouyeh Masoud
MSc, Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, postal code: 1985717411, Iran.
MD, Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, postal code: 1985717413, Iran.
Germs. 2023 Sep 30;13(3):238-253. doi: 10.18683/germs.2023.1390. eCollection 2023 Sep.
The involvement of bacteria in the pathogenesis of biliary tract disease is largely unknown. In this study, we investigated the microbiota of the biliary tissue among adult patients with choledocholithiasis during endoscopic retrograde cholangiography (ERCP).
sequencing of bile samples, culture, and data of the medication history, underlying diseases, and liver function tests were used for the interpretation of differences in the composition of detected bacterial taxa.
The four most common phyla in the bile samples included , , and . Infection with anaerobic and microaerophilic bacteria showed host specificity, where , , , , , and coexist in the same patients. and spp. were detected in 80% and 86% of the patients, where the highest relative abundance rates were detected in patients with elevated alkaline phosphatase (ALP) levels and leukocytosis, respectively. Higher diversity in the bacterial population was detected in patients with common bile duct (CBD) stone, in which the richness of an unclassified member of plus , / spp., , , and s were detected.
Our findings suggested correlations between the presence and relative abundance of several bacterial taxa and CBD stone formation and the effect of medication and underlying diseases on the bile microbial communities. A study on a higher number of bile samples from patients compared with the control group could reveal the role of these bacteria in the pathogenesis of biliary tract disease.
细菌在胆道疾病发病机制中的作用在很大程度上尚不清楚。在本研究中，我们调查了成年胆总管结石患者在进行内镜逆行胰胆管造影（ERCP）期间胆道组织的微生物群。
对胆汁样本进行测序、培养，并结合用药史、基础疾病和肝功能检查数据来解释检测到的细菌类群组成差异。
胆汁样本中最常见的四个门包括 、 、 和 。厌氧菌和微需氧菌感染表现出宿主特异性，其中 、 、 、 、 和 在同一患者中共存。 和 菌分别在80%和86%的患者中检测到，在碱性磷酸酶（ALP）水平升高和白细胞增多的患者中分别检测到最高相对丰度率。在胆总管（CBD）结石患者中检测到细菌种群具有更高的多样性，其中检测到未分类的 成员加上 、 / 菌、 、 、 和 的丰富度。
我们的研究结果表明几种细菌类群的存在和相对丰度与CBD结石形成之间存在相关性，以及药物和基础疾病对胆汁微生物群落的影响。与对照组相比，对更多患者胆汁样本的研究可能揭示这些细菌在胆道疾病发病机制中的作用。
