靶DNA诱导DNA聚合酶活性的转换。
Target DNA induced switches of DNA polymerase activity.
作者信息
Park Ki Soo, Lee Chang Yeol, Park Hyun Gyu
机构信息
Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, Daejeon 305-701, Republic of Korea.
出版信息
Chem Commun (Camb). 2015 Jun 21;51(49):9942-5. doi: 10.1039/c5cc02060c.
PMID:25959533
Abstract
A novel concept that target DNA can induce switching of DNA polymerase activity is devised. The method relies on the finding that a DNA aptamer can undergo conformational change upon hybridization with a complementary target DNA, which leads to activation or inactivation of DNA polymerase. This strategy is utilized to identify the presence of target DNA with high levels of sensitivity and selectivity.
摘要
设计了一种新的概念，即靶DNA可诱导DNA聚合酶活性的转换。该方法基于这样一个发现：DNA适配体与互补靶DNA杂交时会发生构象变化，从而导致DNA聚合酶的激活或失活。利用这一策略可高灵敏度和高选择性地识别靶DNA的存在。
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