基于荧光偏振的核酸检测用于传染病的快速且经济高效诊断
Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.
作者信息
Park Ki Soo, Charles Richelle C, Ryan Edward T, Weissleder Ralph, Lee Hakho
机构信息
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA).
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA).
出版信息
Chemistry. 2015 Nov 9;21(46):16359-63. doi: 10.1002/chem.201502934. Epub 2015 Sep 30.
Abstract
A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
摘要
一种新的核酸检测方法被开发出来用于传染病的快速且经济高效的诊断。这种方法依赖于三个独特的要素：1）检测探针，其根据互补靶标DNA调节DNA聚合酶活性；2）与单一荧光团共轭的通用报告分子；3）荧光偏振（FP）检测。作为概念验证，该检测方法被用于检测沙门氏菌并进行亚型分类，灵敏度可达在不到三小时内检测到单个细菌。
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