Sun Baoyong, Yang Mingshan, Li Min, Wang Fangxin
Department of Bone and Soft Tissue Sarcoma The Shandong Tumor Hospital, Jinan Shandong, 250117, China.
Department of Bone and Soft Tissue Sarcoma The Shandong Tumor Hospital, Jinan Shandong, 250117, China.
Biomed Pharmacother. 2015 Apr;71:58-63. doi: 10.1016/j.biopha.2015.02.014. Epub 2015 Feb 26.
Dysregulation of miRNA is always associated with cancer development and progression. Aberrant expression of miR-217 has been found in some types of cancer. However, its expression and function in osteosarcoma remain unclear. The aim of this study was to explore the effects of miR-217 in osteosarcoma tumorigenesis and development.
The expression level of miR-217 was quantified by real-time RT-PCR in human osteosarcoma cell lines and tissues. MTT, flow cytometric, transwell invasion and migration assays, and tumorigenicity in vivo were adopted to observe the effects of miR-217 on MG-63 cell phenotypes.
MiR-217 was significantly downregulated in osteosarcoma cell lines and clinical specimens. Decreased miR-217 expression was significantly associated with large tumor size, positive distant metastasis, and advanced clinical stage. Low miR-217 expression in osteosarcoma was an independent predictor of poor survival. Overexpression of miR-217 can inhibit the proliferation, invasion, migration and promoted apoptosis of MG-63 cells in vitro and in vivo.
These findings indicate that miR-217 may act as a tumor suppressor in osteosarcoma and would serve as a novel therapeutic agent for miRNA-based therapy.
微小RNA（miRNA）失调总是与癌症的发生和发展相关。在某些类型的癌症中已发现miR-217表达异常。然而，其在骨肉瘤中的表达和功能仍不清楚。本研究旨在探讨miR-217在骨肉瘤发生发展中的作用。
采用实时逆转录聚合酶链反应（RT-PCR）定量检测人骨肉瘤细胞系和组织中miR-217的表达水平。采用MTT法、流式细胞术、Transwell侵袭和迁移实验以及体内致瘤性实验观察miR-217对MG-63细胞表型的影响。
miR-217在骨肉瘤细胞系和临床标本中显著下调。miR-217表达降低与肿瘤体积大、远处转移阳性及临床分期晚显著相关。骨肉瘤中miR-217低表达是生存不良的独立预测因素。miR-217过表达可在体外和体内抑制MG-63细胞的增殖、侵袭、迁移并促进其凋亡。
这些发现表明，miR-217可能在骨肉瘤中起抑癌作用，并有望成为基于miRNA治疗的新型治疗药物。
