Fukuda R, Kohge N, Akagi S, Xuan N T, Tokuda A, Fukumoto S
Second Department of Internal Medicine, Shimane Medical University, Izumo, Japan.
Gastroenterol Jpn. 1993 Apr;28(2):254-8. doi: 10.1007/BF02779228.
To investigate whether hepatitis causes mutation in the viral genome, DNA sequences in the pre-core region of duck hepatitis B virus (DHBV) DNA were analyzed in both ducks with hepatitis and without hepatitis. Five DHBV carrier ducks were injected with DHBV particle proteins purified from duck serum with Freund's complete adjuvant (FCA) intrahepatically from 14 day posthatch for 9 weeks (immunized group). Serum was drawn at the end of the 1st and 4th week after the 1st injection of DHBV particle protein and ducks were killed at the end of the 9th week to obtain the liver. Another five ducks without treatment were used as controls. All ducks of the immunized group showed moderate to severe hepatitis at the 9th week. All ducks in the immunized group showed one mutation except one duck that showed two mutations only at the 9th week. Mutations were observed in the 5th, 13th, 21st, 22nd, and 28th codon of the pre-core region. All of them were point mutation at the 3rd base in the triplets. The frequency of mutation was different in each duck from 20% to 60% but not 100%. There was no mutations in ducks in control group. These results suggest that hepatitis causes mutation in the pre-core lesion genome of duck hepatitis B virus.
为研究肝炎是否会导致病毒基因组发生突变，对患肝炎和未患肝炎的鸭的鸭乙型肝炎病毒（DHBV）DNA前核心区的DNA序列进行了分析。5只DHBV携带鸭从孵化后第14天起，用弗氏完全佐剂（FCA）肝内注射从鸭血清中纯化的DHBV颗粒蛋白，持续9周（免疫组）。在首次注射DHBV颗粒蛋白后的第1周和第4周结束时采集血清，并在第9周结束时处死鸭以获取肝脏。另外5只未经处理的鸭用作对照。免疫组的所有鸭在第9周时均表现出中度至重度肝炎。免疫组的所有鸭除1只在第9周仅出现2个突变外，均出现1个突变。在前核心区的第5、13、21、22和28密码子中观察到突变。所有突变均为三联体中第3个碱基的点突变。每只鸭的突变频率在20%至60%之间，但并非100%。对照组的鸭未出现突变。这些结果表明，肝炎会导致鸭乙型肝炎病毒前核心区基因组发生突变。
