Martins Carla de M, do Nascimento Evandro A, de Morais Sérgio A L, de Oliveira Alberto, Chang Roberto, Cunha Luís C S, Martins Mário M, Martins Carlos Henrique G, Moraes Thaís da S, Rodrigues Paulla V, da Silva Cláudio V, de Aquino Francisco J T
Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil ; Goian Federal Institute, Campus Morrinhos, 75650-000 Morrinhos, GO, Brazil.
Nucleus of Research in Natural Products, Chemistry Institute, Federal University of Uberlândia, 38400-902 Uberlândia, MG, Brazil.
Evid Based Complement Alternat Med. 2015;2015:842047. doi: 10.1155/2015/842047. Epub 2015 Apr 16.
Many essential oils (EOs) of different plant species possess interesting antimicrobial effects on buccal microorganisms and cytotoxic properties. EOs of Kielmeyera coriacea Mart. & Zucc. were analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The EO from leaves is rich in sesquiterpenes hydrocarbons and oxygenated sesquiterpenes. The three major compounds identified were germacrene-D (24.2%), (E)-caryophyllene (15.5%), and bicyclogermacrene (11.6%). The inner bark EO is composed mainly of sesquiterpenes hydrocarbons and the major components are alpha-copaene (14.9%) and alpha-(E)-bergamotene (13.0%). The outer bark EO is composed mainly of oxygenated sesquiterpenes and long-chain alkanes, and the major components are alpha-eudesmol (4.2%) and nonacosane (5.8%). The wood EO is mainly composed of long-chain alkanes and fatty acids, and the major components are nonacosane (9.7%) and palmitic acid (16.2%). The inner bark EO showed the strongest antimicrobial activity against the anaerobic bacteria Prevotella nigrescens (minimum inhibitory concentration-MIC of 50 µg mL(-1)). The outer bark and wood EOs showed MICs of 100 µg mL(-1) for all aerobic microorganisms tested. The EOs presented low toxicity to Vero cells. These results suggest that K. coriacea, a Brazilian plant, provide initial evidence of a new and alternative source of substances with medicinal interest.
许多不同植物物种的精油（EOs）对口腔微生物具有有趣的抗菌作用和细胞毒性特性。对 Kielmeyera coriacea Mart. & Zucc. 的精油进行了气相色谱-质谱联用（GC-MS）分析。叶精油富含倍半萜烃类和氧化倍半萜类。鉴定出的三种主要化合物为杜松烯-D（24.2%）、（E）-石竹烯（15.5%）和双环杜松烯（11.6%）。内皮精油主要由倍半萜烃类组成，主要成分是α-可巴烯（14.9%）和α-（E）-佛手柑烯（13.0%）。外皮精油主要由氧化倍半萜类和长链烷烃组成，主要成分是α-桉叶醇（4.2%）和二十九烷（5.8%）。木材精油主要由长链烷烃和脂肪酸组成，主要成分是二十九烷（9.7%）和棕榈酸（16.2%）。内皮精油对厌氧细菌变黑普雷沃菌显示出最强的抗菌活性（最低抑菌浓度-MIC为50μg mL-1）。外皮和木材精油对所有测试的需氧微生物的MIC均为100μg mL-1。这些精油对Vero细胞毒性较低。这些结果表明，巴西植物 Kielmeyera coriacea 为具有药用价值的新的替代物质来源提供了初步证据。
