Martinović Rajko, Kolarević Stoimir, Kračun-Kolarević Margareta, Kostić Jovana, Marković Sandra, Gačić Zoran, Kljajić Zoran, Vuković-Gačić Branka
Institute of Marine Biology - Kotor, University of Montenegro, Dobrota bb, 85330, Kotor, Montenegro.
Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, Studentski trg 16, University of Belgrade, Belgrade, Serbia.
Mar Environ Res. 2015 Jul;108:83-90. doi: 10.1016/j.marenvres.2015.05.001. Epub 2015 May 7.
The effects of ex situ exposure of Mytilus galloprovincialis to Superdispersant-25 (S-25), diesel oil and dispersed diesel oil mixtures were studied by the impact on level of DNA damage in haemocytes (comet assay) and the cardiac activity patterns of mussels. Specimens were exposed for 72 h in a static system to diesel oil (100 μL/L and 1 mL/L), S-25 (5 and 50 μL/L), and dispersed diesel oil mixtures M1 (diesel oil 100 μL/L + S-25 5 μL/L) and M2 (diesel oil 1 mL/L + S-25 50 μL/L). For positive control 40 μM CdCl2 was used. The comet assay results indicated genotoxic potential of S-25 while the effects of diesel oil alone were not observed. The highest response was detected for M1 while the effects of M2 were not detected. The heart rate disorders were recorded for the diesel oil (1 mL/L), S-25 (50 μL/L) and both dispersed diesel oil mixtures.
通过对血细胞中DNA损伤水平（彗星试验）和贻贝心脏活动模式的影响，研究了地中海贻贝异地暴露于超级分散剂-25（S-25）、柴油和分散柴油混合物的效应。将样本在静态系统中暴露72小时，分别接触柴油（100μL/L和1mL/L）、S-25（5和50μL/L）以及分散柴油混合物M1（柴油100μL/L + S-25 5μL/L）和M2（柴油1mL/L + S-25 50μL/L）。以40μM CdCl2作为阳性对照。彗星试验结果表明S-25具有遗传毒性潜力，而未观察到单独柴油的影响。对M1检测到最高反应，未检测到M2的影响。记录到柴油（1mL/L）、S-25（50μL/L）以及两种分散柴油混合物均出现心率紊乱。
