Shnyreva A A, Shnyreva A V
Genetika. 2015 Feb;51(2):177-87.
We performed phylogenetic analysis for ten Pleurotus species, based on internal transcribed spacer (ITS) sequences of rDNA. A phylogenetic tree was constructed on the basis of 31 oyster fungi strains of different origin and 10 reference sequences from GenBank. Our analysis demonstrates that the tested Pleurotus species are of monophyletic origin. We evaluated the evolutionary distances between these species. Classic genetic analysis of sexual compatibility based on monocaryon (mon)-mon crosses showed no reproductive barriers within the P. cornucopiae-P. euosmus species complex. Thus, despite the divergence (subclustering) between commercial strains and natural isolates of P. ostreatus revealed by phylogenetic analysis, there is no reproductive isolation between these groups. A common allele of the matB locus was identified for the commercial strains Sommer and L/4, supporting the common origin of these strains.
我们基于核糖体DNA的内转录间隔区(ITS)序列,对十种侧耳属物种进行了系统发育分析。根据31株不同来源的平菇菌株和来自GenBank的10条参考序列构建了系统发育树。我们的分析表明,所测试的侧耳属物种起源于单系。我们评估了这些物种之间的进化距离。基于单核体(mon)-单核体杂交的性亲和性经典遗传分析表明,在白黄侧耳-真美味侧耳物种复合体中不存在生殖障碍。因此,尽管系统发育分析揭示了糙皮侧耳商业菌株与自然分离株之间的差异(亚聚类),但这些群体之间不存在生殖隔离。为商业菌株Sommer和L/4鉴定出了matB基因座的一个共同等位基因,支持了这些菌株的共同起源。