Wang Peng, Ren Dunlin, Chen Ying, Jiang Meiju, Wang Robin, Wang Yan-Gang
Department of Endocrinology, Chengyang People's Hospital of Qingdao City, Qingdao, China.
Cell Physiol Biochem. 2015;36(1):201-7. doi: 10.1159/000374064. Epub 2015 Apr 30.
Resveratrol has been shown to exert anti-inflammatory and antioxidant effects, while sodium alginate is a common pharmaceutic adjuvant with antioxidative and immunomodulatory properties. We performed an animal study to investigate the effect of sodium alginate addition to resveratrol on acute gouty arthritis.
Twenty-four SPF Wistar mice were randomized to four groups receiving the combination of sodium alginate and resveratrol, resveratrol alone, colchicine, and placebo, respectively. Acute gouty arthritis was induced by injection of 0.05 ml monosodium urate (MSU) solution (25g/mL) into ankle joint cavity. IL-1β, CCR5, and CXCL10 levels in both serum and synovial fluid were measured using ELISA. NLRP3 expression in the synovial tissues was measured using western plot.
The combination of sodium alginate and resveratrol significantly reduced synovial levels of IL-1β, CCR5, and CXCL10 when compared with colchicines, and all P values were less than 0.0001. The combination of sodium alginate and resveratrol was also superior to resveratrol in terms of both serum levels and synovial levels of IL-1β, CCR5, and CXCL10. In addition, resveratrol, with or without sodium alginate, could reduce NLRP3 expression obviously in the synovial tissues.
The combination of sodium alginate and resveratrol has better effect over colchicines in treating MSU-induced acute gouty arthritis.
白藜芦醇已被证明具有抗炎和抗氧化作用，而海藻酸钠是一种具有抗氧化和免疫调节特性的常见药物佐剂。我们进行了一项动物研究，以探讨在白藜芦醇中添加海藻酸钠对急性痛风性关节炎的影响。
将24只SPF级Wistar小鼠随机分为四组，分别接受海藻酸钠与白藜芦醇联合用药、单独使用白藜芦醇、秋水仙碱和安慰剂。通过向踝关节腔注射0.05 ml尿酸钠（MSU）溶液（25g/mL）诱导急性痛风性关节炎。使用酶联免疫吸附测定法（ELISA）测量血清和滑液中IL-1β、CCR5和CXCL10的水平。使用蛋白质免疫印迹法测量滑膜组织中NLRP3的表达。
与秋水仙碱相比，海藻酸钠和白藜芦醇的组合显著降低了滑膜中IL-1β、CCR5和CXCL10的水平，所有P值均小于0.0001。在IL-1β、CCR5和CXCL10的血清水平和滑膜水平方面，海藻酸钠和白藜芦醇的组合也优于白藜芦醇。此外，无论有无海藻酸钠，白藜芦醇均可明显降低滑膜组织中NLRP3的表达。
在治疗MSU诱导的急性痛风性关节炎方面，海藻酸钠和白藜芦醇的组合比秋水仙碱具有更好的效果。
