Key Laboratory of Biotechnology and Bioresources Utilization (Ministry of Education), College of Life Science, Dalian Minzu University, Dalian 116600, China.
Int J Mol Sci. 2022 Oct 8;23(19):11953. doi: 10.3390/ijms231911953.
Selenium nanoparticles (SeNPs) are all important for research because they exhibit a higher degree of absorption and lower toxicity than that of their organic and inorganic forms. At present, there are few reports on marine strains that can reduce Se(IV) to generate Se(0). In this study, a strain that reduces sodium selenite to SeNPs with high efficiency was screened from 40 marine strains. The SeNPs-S produced by the whole cells and SeNPs-E produced by the extracellular extract were characterized by FTIR, UV, Raman, XRD and SEM. Based on the results, the two kinds of SeNPs exhibited obvious differences in morphology, and their surfaces were capped with different biomacromolecules. Due to the difference in shape and surface coating, opposite results were obtained for the antibacterial activity of SeNPs-S and SeNPs-E against Gram-positive and Gram-negative bacteria. Both SeNPs-S and SeNPs-E exhibited no obvious cytotoxicity at concentrations up to 100 μg/mL, but SeNPs-E retained lower cytotoxicity when its concentration increased to 200 μg/mL. This is the first report on the detailed difference between the SeNPs produced by whole cells and cell extracts.
硒纳米颗粒（SeNPs）在研究中非常重要，因为它们表现出更高的吸收率和更低的毒性，优于其有机和无机形式。目前，关于能够将硒（IV）还原为硒（0）的海洋菌株的报道很少。在本研究中，从 40 株海洋菌株中筛选出一种能够高效地将亚硒酸钠还原为 SeNPs 的菌株。通过 FTIR、UV、Raman、XRD 和 SEM 对全细胞产生的 SeNPs-S 和胞外提取物产生的 SeNPs-E 进行了表征。基于这些结果，两种 SeNPs 在形态上表现出明显的差异，其表面覆盖着不同的生物大分子。由于形状和表面涂层的差异，SeNPs-S 和 SeNPs-E 对革兰氏阳性菌和革兰氏阴性菌的抗菌活性得到了相反的结果。在浓度高达 100μg/mL 时，SeNPs-S 和 SeNPs-E 均没有明显的细胞毒性，但当浓度增加到 200μg/mL 时，SeNPs-E 保持较低的细胞毒性。这是首次详细报道了全细胞和细胞提取物产生的 SeNPs 之间的差异。
