Research Center "PhytoEngineering" Ltd., 141880 Rogachevo, Moscow Region, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow, Russia.
Viruses. 2021 Jun 8;13(6):1095. doi: 10.3390/v13061095.
Using bacteriophages (bacterial viruses) to control pathogenic bacteria is a promising approach in horticulture. However, the application of this strategy in real conditions requires compliance with particular technological and environmental restraints. The presented paper concerns the process of phage selection to create a cocktail that is efficient against the circulating causal agents of potato soft rot. The resulting phage cocktail causes a complete lysis of a mixture of circulating pectobacterial strains in vitro. In the context of being used to treat ware potatoes during off-season storage, the protocol of phage application via the humidity maintenance system was designed. The phage cocktail was shown to reduce the population of spp. 10-12-fold, achieving a population that was below a symptomatic threshold.
利用噬菌体(细菌病毒)来控制致病菌是园艺学中一种很有前途的方法。然而,该策略在实际条件下的应用需要符合特定的技术和环境限制。本文涉及到选择噬菌体以创建一种鸡尾酒的过程,这种鸡尾酒可有效对抗马铃薯软腐病的循环病原体。所得到的噬菌体鸡尾酒可在体外完全裂解循环果胶杆菌菌株的混合物。在用于淡季贮藏期间对商品马铃薯进行处理时,通过湿度维持系统应用噬菌体的方案得到了设计。噬菌体鸡尾酒被证明可使 spp. 的种群减少 10-12 倍,使种群数量低于症状阈值。