†Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China.
‡National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Institute of Microelectronics, Peking University, Beijing 100871, China.
Anal Chem. 2015 Jun 16;87(12):6397-402. doi: 10.1021/acs.analchem.5b01440. Epub 2015 May 26.
Herein, we proposed a portable, easy-to-operate, and antifouling microcapsule array chip for target detection. This prepackaged chip was fabricated by innovative and cost-effective 3D ice printing integrating with photopolymerization sealing which could eliminate complicated preparation of wet chemistry and effectively resist outside contaminants. Only a small volume of sample (2 μL for each microcapsule) was consumed to fulfill the assay. All the reagents required for the analysis were stored in ice form within the microcapsule before use, which guaranteed the long-term stability of microcapsule array chips. Nitrite and glucose were chosen as models for proof of concept to achieve an instant quantitative detection by naked eyes without the need of external sophisticated instruments. The simplicity, low cost, and small sample consumption endowed ice-printing microcapsule array chips with potential commercial value in the fields of on-site environmental monitoring, medical diagnostics, and rapid high-throughput point-of-care quantitative assay.
在这里，我们提出了一种用于目标检测的便携式、易于操作且抗污染的微胶囊阵列芯片。这种预制芯片是通过创新且具有成本效益的 3D 冰打印技术与光聚合密封技术集成制造而成，可消除繁琐的湿法化学制备过程，并有效抵抗外部污染物。该芯片仅消耗少量样品（每个微胶囊 2 μL）即可完成检测。在使用前，所有用于分析的试剂都以冰的形式储存在微胶囊内，从而保证了微胶囊阵列芯片的长期稳定性。亚硝酸盐和葡萄糖被选为概念验证的模型，通过肉眼即可实现即时定量检测，无需外部复杂仪器。这种简单、低成本和小样本消耗的特性使得冰印微胶囊阵列芯片在现场环境监测、医学诊断和快速高通量即时检测等领域具有潜在的商业价值。
