He Enqi, Cao Ting, Cai Liangyuan, Guo Dan, Zhou Yinglin, Zhang Xinxiang, Li Zhihong
State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University Beijing 100084 China.
Center for Nano and Micro Mechanics, Tsinghua University Beijing 100084 China.
RSC Adv. 2018 Nov 27;8(69):39561-39566. doi: 10.1039/c8ra07045h. eCollection 2018 Nov 23.
In this work, a novel microcapsule array chip was fabricated for the detection of Salmonella DNA by integrating an ice-printing technique with DNA isothermal amplification. Reaction solutions were previously sealed in the microcapsule array chip ice printing. To protect the relatively fragile DNA isothermal amplification system, an extra polystyrene (PS) film was introduced to isolate the reaction solution from photopolymer precursor, which was proved to be a vital step for providing a clean and stable environment for DNA amplification reaction. Detection operation can be done by simply injecting sample DNA into the microcapsule by an easily accessible syringe, and the result can be directly obtained through color change within 90 minutes. This method shows good sensitivity, specificity and stability.
在这项工作中，通过将冰印技术与DNA等温扩增相结合，制备了一种用于检测沙门氏菌DNA的新型微胶囊阵列芯片。反应溶液预先在微胶囊阵列芯片中通过冰印密封。为了保护相对脆弱的DNA等温扩增系统，引入了额外的聚苯乙烯（PS）膜，以将反应溶液与光聚合物前体隔离，这被证明是为DNA扩增反应提供清洁稳定环境的关键步骤。检测操作可以通过用易于操作的注射器将样品DNA简单地注入微胶囊来完成，并且可以在90分钟内通过颜色变化直接获得结果。该方法具有良好的灵敏度、特异性和稳定性。
