Barua A B, Olson J A
Am J Clin Nutr. 1986 Apr;43(4):481-5. doi: 10.1093/ajcn/43.4.481.
The occurrence of retinoyl beta-glucuronide and retinoic acid as normal endogenous components of vitamin A reserve in human blood has been demonstrated. Use of high-performance liquid chromatography, coupled with a sensitive detector and integrator, has enabled us to quantitate nanogram quantities of the two retinoids. Serum concentrations of retinoyl glucuronide and retinoic acid in all the volunteers studied ranged from 1.5 to 5.1 ng/ml (mean 2.42 ng/ml) and from 1.0 to 3.2 ng/ml (mean 1.80 ng/ml) serum, respectively. The identity of retinoyl beta-glucuronide was confirmed by its conversion to retinoic acid by the action of beta-glucuronidase and by study of the mass spectrum of the methylated derivative.
已证实视黄酰β-葡萄糖醛酸酯和视黄酸作为人体血液中维生素A储备的正常内源性成分存在。使用高效液相色谱法，结合灵敏的检测器和积分仪，使我们能够对纳克量的这两种类视黄醇进行定量。在所有研究的志愿者中，血清视黄酰葡萄糖醛酸酯和视黄酸的浓度分别为1.5至5.1纳克/毫升（平均2.42纳克/毫升）和1.0至3.2纳克/毫升（平均1.80纳克/毫升）血清。视黄酰β-葡萄糖醛酸酯通过β-葡萄糖醛酸酶作用转化为视黄酸以及通过对甲基化衍生物的质谱研究得以确认。
