Zhang Bo, Yan Lijuan, Li Qiang, Zou Jie, Tan Hao, Tan Wei, Peng Weihong, Li Xiaolin, Zhang Xiaoping
Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
PeerJ. 2018 Jun 13;6:e4975. doi: 10.7717/peerj.4975. eCollection 2018.
, a valuable medicinal fungus, is widely distributed in China. It grows alongside with a complex microbial ecosystem in the substrate. As sequencing technology advances, it is possible to reveal the composition and functions of substrate-associated bacterial communities.
We analyzed the bacterial community dynamics in the substrate during the four typical growth stages of using next-generation sequencing.
The physicochemical properties of the substrate (e.g. acidity, moisture, total nitrogen, total phosphorus and total potassium) changed between different growth stages. A total of 598,771 sequences from 12 samples were obtained and assigned to 22 bacterial phyla. and were the dominant phyla. Bacterial community composition and diversity significantly differed between the elongation stage and the other three growth stages. LEfSe analysis revealed a large number of bacterial taxa (e.g. , and ) with significantly higher abundance at the elongation stage. Functional pathway prediction uncovered significant abundance changes of a number of bacterial functional pathways between the elongation stage and other growth stages. At the elongation stage, the abundance of the environmental information processing pathway (mainly membrane transport) decreased, whereas that of the metabolism-related pathways increased.
The changes in bacterial community composition, diversity and predicted functions were most likely related to the changes in the moisture and nutrient conditions in the substrate with the growth of , particularly at the elongation stage. Our findings shed light on the -bacteria-substrate relationships, which should facilitate the industrial cultivation of .
[某种珍贵药用真菌名称]是一种珍贵的药用真菌，在中国广泛分布。它与基质中复杂的微生物生态系统共生生长。随着测序技术的发展，揭示基质相关细菌群落的组成和功能成为可能。
我们使用下一代测序技术分析了[该药用真菌名称]四个典型生长阶段基质中的细菌群落动态。
不同生长阶段基质的理化性质（如酸度、湿度、全氮、全磷和全钾）发生了变化。从12个样本中总共获得了598,771条序列，并归类到22个细菌门。[两个细菌门名称]是优势门。伸长阶段与其他三个生长阶段的细菌群落组成和多样性存在显著差异。线性判别分析效应大小（LEfSe）分析显示，在伸长阶段有大量细菌分类群（如[一些细菌分类群名称]）的丰度显著更高。功能途径预测发现伸长阶段与其他生长阶段之间许多细菌功能途径的丰度有显著变化。在伸长阶段，环境信息处理途径（主要是膜运输）的丰度下降，而与代谢相关途径的丰度增加。
细菌群落组成、多样性和预测功能的变化很可能与随着[该药用真菌名称]生长基质中水分和养分条件的变化有关，特别是在伸长阶段。我们的研究结果揭示了[该药用真菌名称]-细菌-基质之间的关系，这将有助于[该药用真菌名称]的工业化栽培。
