蜡样芽孢杆菌群九个新物种的提议。
Proposal of nine novel species of the Bacillus cereus group.
作者信息
Liu Yang, Du Juan, Lai Qiliang, Zeng Runying, Ye Dezan, Xu Jun, Shao Zongze
机构信息
State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Institute of Oceanology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
出版信息
Int J Syst Evol Microbiol. 2017 Aug;67(8):2499-2508. doi: 10.1099/ijsem.0.001821. Epub 2017 Aug 10.
Nine novel Gram-stain-positive bacteria were investigated by a polyphasic taxonomic approach. Based on the analysis of 16S rRNA gene sequences, these strains belonged to the Bacillus cereus group, sharing over 97 % similarity with the known species of this group, and less than 95 % similarity with other species of the genus Bacillus. Multilocus sequence typing analysis showed that they formed nine robust and well-separated branches from the known species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the nine strains were, respectively, below the 70 and 96 % threshold values for species definition, and between each strain and the known type strains of this group were also below the two threshold values. On the basis of the phenotypic and phylogenetic data, along with low dDDH and ANI values among these strains, these bacteria are assigned to the following nine novel species of the B. cereus group: Bacillus paranthracis sp. nov., type strain Mn5T (=MCCC 1A00395T=KCTC 33714T=LMG 28873T); Bacillus pacificus sp. nov., type strain EB422T (=MCCC 1A06182T=KCTC 33858T); Bacillus tropicus sp. nov., type strain N24T (=MCCC 1A01406T=KCTC 33711T=LMG 28874T); Bacillus albus sp. nov., type strain N35-10-2T (=MCCC 1A02146T=KCTC 33710T=LMG 28875T); Bacillus mobilis sp. nov., type strain 0711P9-1T (=MCCC 1A05942T=KCTC 33717T=LMG 28877T); Bacillus luti sp. nov., type strain TD41T (=MCCC 1A00359T=KCTC 33716T=LMG 28872T); Bacillus proteolyticus sp. nov., type strain TD42T (=MCCC 1A00365T=KCTC 33715T=LMG 28870T); Bacillus nitratireducens sp. nov., type strain 4049T (=MCCC 1A00732T=KCTC 33713T=LMG 28871T); and Bacillus paramycoides sp. nov., type strain NH24A2T (=MCCC 1A04098T=KCTC 33709T=LMG 28876T).
采用多相分类方法对9株新的革兰氏阳性菌进行了研究。基于16S rRNA基因序列分析，这些菌株属于蜡样芽孢杆菌群，与该群已知物种的相似度超过97%，与芽孢杆菌属其他物种的相似度低于95%。多位点序列分型分析表明，它们与已知物种形成了9个稳健且分离良好的分支。这9株菌株之间的数字DNA-DNA杂交（dDDH）值和平均核苷酸同一性（ANI）值分别低于物种定义的70%和96%阈值，并且每株菌株与该群已知模式菌株之间的值也低于这两个阈值。根据表型和系统发育数据，以及这些菌株之间较低的dDDH和ANI值，这些细菌被归为蜡样芽孢杆菌群的以下9个新物种：新种炭疽芽孢杆菌（Bacillus paranthracis sp. nov.），模式菌株Mn5T（=MCCC 1A00395T=KCTC 33714T=LMG 28873T）；新种太平洋芽孢杆菌（Bacillus pacificus sp. nov.），模式菌株EB422T（=MCCC 1A06182T=KCTC 33858T）；新种热带芽孢杆菌（Bacillus tropicus sp. nov.），模式菌株N24T（=MCCC 1A01406T=KCTC 33711T=LMG 28874T）；新种白色芽孢杆菌（Bacillus albus sp. nov.），模式菌株N35-10-2T（=MCCC 1A02146T=KCTC 33710T=LMG 28875T）；新种运动芽孢杆菌（Bacillus mobilis sp. nov.），模式菌株0711P9-1T（=MCCC 1A05942T=KCTC 33717T=LMG 28877T）；新种泥芽孢杆菌（Bacillus luti sp. nov.），模式菌株TD41T（=MCCC 1A00359T=KCTC 33716T=LMG 28872T）；新种蛋白水解芽孢杆菌（Bacillus proteolyticus sp. nov.），模式菌株TD42T（=MCCC 1A00365T=KCTC 33715T=LMG 28870T）；新种硝酸盐还原芽孢杆菌（Bacillus nitratireducens sp. nov.），模式菌株4049T（=MCCC 1A00732T=KCTC 33713T=LMG 28871T）；以及新种类蕈状芽孢杆菌（Bacillus paramycoides sp. nov.），模式菌株NH24A2T（=MCCC 1A04098T=KCTC 33709T=LMG 28876T）。
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