Liu Yang, Lai Qiliang, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2018 Jan;68(1):106-112. doi: 10.1099/ijsem.0.002466. Epub 2017 Nov 2.
The aim of this study was to clarify the taxonomic status of the species Bacillus weihenstephanensis. A complete genome sequence for the type strain of B. weihenstephanensis was compared against that of the closely related type strain of Bacillus mycoides. The digital DNA-DNA hybridization and average nucleotide identity values between the two type strains was greater than two recognized thresholds for bacterial species delineation, indicating that they should belong to the same genomospecies. The psychrotolerant characteristic and signature sequences of 16S rRNA and cspA genes were incapable of distinguishing B. weihenstephanensis from some non-B. weihenstephanensis strains. Meanwhile, the metabolic, physiological and chemotaxonomic features for the type strain of B. weihenstephanensis were shown to be congruent with those of B. mycoides. On this basis, the taxonomic affiliations of related strains from the Genbank database were determined using multilocus sequence typing and genomic analyses. Therefore, we propose Bacillus weihenstephanensis as a later heterotypic synonym of Bacillus mycoides and correction of erroneous species identifications for several strains.
本研究的目的是阐明魏氏芽孢杆菌（Bacillus weihenstephanensis）的分类地位。将魏氏芽孢杆菌模式菌株的完整基因组序列与密切相关的蕈状芽孢杆菌（Bacillus mycoides）模式菌株的基因组序列进行比较。两株模式菌株之间的数字DNA-DNA杂交和平均核苷酸同一性值大于细菌物种划分的两个公认阈值，表明它们应属于同一基因组种。耐冷特性以及16S rRNA和cspA基因的特征序列无法区分魏氏芽孢杆菌与一些非魏氏芽孢杆菌菌株。同时，魏氏芽孢杆菌模式菌株的代谢、生理和化学分类特征与蕈状芽孢杆菌的特征一致。在此基础上，利用多位点序列分型和基因组分析确定了Genbank数据库中相关菌株的分类归属。因此，我们提议将魏氏芽孢杆菌作为蕈状芽孢杆菌的晚出异名，并对几株菌株的错误物种鉴定进行修正。
