Di Caprio Roberta, Lembo Serena, Di Costanzo Luisa, Balato Anna, Monfrecola Giuseppe
Department of Clinical Medicine and Surgery, Section of Dermatology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
Mediators Inflamm. 2015;2015:329418. doi: 10.1155/2015/329418. Epub 2015 Apr 22.
Doxycycline is used to treat infective diseases because of its broadspectrum efficacy. High dose administration (100 or 200 mg/day) is often responsible for development of bacterial resistances and endogenous flora alterations, whereas low doses (20-40 mg/day) do not alter bacteria susceptibility to antibiotics and exert anti-inflammatory activities. In this study, we wanted to assess the efficacy of both low and high doxycycline doses in modulating IL-8, TNF-α, and IL-6 gene expression in HaCaT cells stimulated with LPS. Three experimental settings were used, differing in the timing of doxycycline treatment in respect to the insult induced by LPS: pretreatment, concomitant, and posttreatment. Low doses were more effective than high doses in modulating gene expression of LPS-induced proinflammatory cytokines (IL-8, TNF-α, and IL-6), when added before (pretreatment) or after (posttreatment) LPS stimulation. This effect was not appreciated when LPS and doxycycline were simultaneously added to cell cultures: in this case high doses were more effective. In conclusion, our in vitro study suggests that low doxycycline doses could be safely used in chronic or acute skin diseases in which the inflammatory process, either constantly in progress or periodically recurring, has to be prevented or controlled.
强力霉素因其广谱疗效而被用于治疗感染性疾病。高剂量给药（100或200毫克/天）往往会导致细菌耐药性的产生和内源性菌群的改变，而低剂量（20 - 40毫克/天）则不会改变细菌对抗生素的敏感性，并具有抗炎活性。在本研究中，我们想评估低剂量和高剂量强力霉素在调节脂多糖（LPS）刺激的HaCaT细胞中白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）基因表达方面的疗效。我们采用了三种实验设置，强力霉素治疗的时间相对于LPS诱导的损伤有所不同：预处理、同时处理和后处理。当在LPS刺激之前（预处理）或之后（后处理）添加时，低剂量在调节LPS诱导的促炎细胞因子（IL-8、TNF-α和IL-6）的基因表达方面比高剂量更有效。当LPS和强力霉素同时添加到细胞培养物中时，这种效果并不明显：在这种情况下，高剂量更有效。总之，我们的体外研究表明，低剂量强力霉素可安全用于慢性或急性皮肤病，在这些疾病中，无论是持续进行还是周期性复发的炎症过程都必须得到预防或控制。
