J. Craig Venter Institute , La Jolla, CA 92037, USA.
Northrop Grumman Information Systems, Health IT , Rockville, MD 20850, USA.
Sci Data. 2014 Oct 14;1:140033. doi: 10.1038/sdata.2014.33. eCollection 2014.
The Systems Biology for Infectious Diseases Research program was established by the U.S. National Institute of Allergy and Infectious Diseases to investigate host-pathogen interactions at a systems level. This program generated 47 transcriptomic and proteomic datasets from 30 studies that investigate in vivo and in vitro host responses to viral infections. Human pathogens in the Orthomyxoviridae and Coronaviridae families, especially pandemic H1N1 and avian H5N1 influenza A viruses and severe acute respiratory syndrome coronavirus (SARS-CoV), were investigated. Study validation was demonstrated via experimental quality control measures and meta-analysis of independent experiments performed under similar conditions. Primary assay results are archived at the GEO and PeptideAtlas public repositories, while processed statistical results together with standardized metadata are publically available at the Influenza Research Database (www.fludb.org) and the Virus Pathogen Resource (www.viprbrc.org). By comparing data from mutant versus wild-type virus and host strains, RNA versus protein differential expression, and infection with genetically similar strains, these data can be used to further investigate genetic and physiological determinants of host responses to viral infection.
传染病系统生物学研究计划由美国国家过敏和传染病研究所设立，旨在从系统水平上研究宿主-病原体相互作用。该计划从 30 项研究中生成了 47 个转录组学和蛋白质组学数据集，这些研究调查了病毒感染的体内和体外宿主反应。研究对象包括正粘病毒科和冠状病毒科的人类病原体，特别是大流行性 H1N1 和禽流感 H5N1 甲型流感病毒以及严重急性呼吸综合征冠状病毒（SARS-CoV）。通过实验质量控制措施和在类似条件下进行的独立实验的荟萃分析来证明研究验证。主要测定结果被存档在 GEO 和 PeptideAtlas 公共存储库中，而处理后的统计结果和标准化元数据则可在流感研究数据库（www.fludb.org）和病毒病原体资源库（www.viprbrc.org）上公开获取。通过比较突变体与野生型病毒和宿主株、RNA 与蛋白质差异表达以及遗传相似株的感染，这些数据可用于进一步研究宿主对病毒感染反应的遗传和生理决定因素。
