Bonnin Rémy A, Bouloc Philippe
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91400 Orsay, France.
Int J Genomics. 2015;2015:395753. doi: 10.1155/2015/395753. Epub 2015 Apr 21.
The regulation of RNA decay is now widely recognized as having a central role in bacterial adaption to environmental stress. Here we present an overview on the diversity of ribonucleases (RNases) and their impact at the posttranscriptional level in the human pathogen Staphylococcus aureus. RNases in prokaryotes have been mainly studied in the two model organisms Escherichia coli and Bacillus subtilis. Based on identified RNases in these two models, putative orthologs have been identified in S. aureus. The main staphylococcal RNases involved in the processing and degradation of the bulk RNA are (i) endonucleases RNase III and RNase Y and (ii) exonucleases RNase J1/J2 and PNPase, having 5' to 3' and 3' to 5' activities, respectively. The diversity and potential roles of each RNase and of Hfq and RppH are discussed in the context of recent studies, some of which are based on next-generation sequencing technology.
RNA 衰变的调控如今被广泛认为在细菌适应环境压力方面发挥着核心作用。在此，我们概述了核糖核酸酶（RNase）的多样性及其在人类病原体金黄色葡萄球菌转录后水平上的影响。原核生物中的核糖核酸酶主要是在两种模式生物大肠杆菌和枯草芽孢杆菌中进行研究的。基于在这两种模式生物中已鉴定出的核糖核酸酶，在金黄色葡萄球菌中也鉴定出了假定的直系同源物。参与大量 RNA 加工和降解的主要葡萄球菌核糖核酸酶有：（i）内切核酸酶 RNase III 和 RNase Y，以及（ii）外切核酸酶 RNase J1/J2 和 PNPase，它们分别具有 5' 到 3' 和 3' 到 5' 的活性。结合近期的研究（其中一些基于新一代测序技术），讨论了每种核糖核酸酶以及 Hfq 和 RppH 的多样性和潜在作用。
