• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

植物病理学中镰刀菌属孢子悬浮液定量快速方法的调整。

Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology.

作者信息

Caligiore-Gei Pablo F, Valdez Jorge G

机构信息

INTA EEA La Consulta, La Consulta, Mendoza, Argentina.

INTA EEA La Consulta, La Consulta, Mendoza, Argentina.

出版信息

Rev Argent Microbiol. 2015 Apr-Jun;47(2):152-4. doi: 10.1016/j.ram.2015.03.002. Epub 2015 May 12.

DOI:10.1016/j.ram.2015.03.002
PMID:25979149
Abstract

The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions of isolates of the genus Fusarium. In this work we present a linear formula to relate absorbance measurements at 530nm with the number of microconidia/ml in a suspension.

摘要

当需要对细胞悬液进行定量时，使用血细胞计数板是一种广泛采用的方法。然而，这种技术可能耗时较长且需要训练有素的人员。分光光度法已被证明是一种快速、简单且准确的方法，用于估计镰刀菌属分离株孢子悬液的浓度。在这项工作中，我们提出了一个线性公式，用于将530nm处的吸光度测量值与悬液中每毫升微分生孢子的数量相关联。

相似文献

1
Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology.植物病理学中镰刀菌属孢子悬浮液定量快速方法的调整。
Rev Argent Microbiol. 2015 Apr-Jun;47(2):152-4. doi: 10.1016/j.ram.2015.03.002. Epub 2015 May 12.
2
Detection and quantification of airborne conidia of Fusarium circinatum, the causal agent of pine pitch canker, from two California sites by using a real-time PCR approach combined with a simple spore trapping method.通过将实时荧光定量PCR方法与一种简单的孢子捕获方法相结合，对来自加利福尼亚两个地点的引起松材溃疡病的病原菌——轮状镰刀菌的气传分生孢子进行检测和定量分析。
Appl Environ Microbiol. 2004 Jun;70(6):3512-20. doi: 10.1128/AEM.70.6.3512-3520.2004.
3
Canker and wilt of black locust (Robinia pseudoacacia L.) caused by Fusarium species.由镰刀菌属物种引起的刺槐溃疡病和枯萎病。
Acta Microbiol Immunol Hung. 2002;49(2-3):249-60. doi: 10.1556/AMicr.49.2002.2-3.11.
4
Molecular detection of Fusarium oxysporum f. sp. niveum and Mycosphaerella melonis in infected plant tissues and soil.在受感染植物组织和土壤中尖孢镰刀菌西瓜专化型和瓜球腔菌的分子检测
FEMS Microbiol Lett. 2005 Aug 1;249(1):39-47. doi: 10.1016/j.femsle.2005.05.057.
5
Prospects of molecular markers in Fusarium species diversity.镰刀菌物种多样性中分子标记的前景。
Appl Microbiol Biotechnol. 2011 Jun;90(5):1625-39. doi: 10.1007/s00253-011-3209-3. Epub 2011 Apr 15.
6
The ITS region as a taxonomic discriminator between Fusarium verticillioides and Fusarium proliferatum.作为轮枝镰孢菌和层出镰孢菌分类鉴别特征的内转录间隔区。
Mycol Res. 2009 Oct;113(Pt 10):1137-45. doi: 10.1016/j.mycres.2009.07.011. Epub 2009 Jul 23.
7
Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan.建立台湾地区尖孢镰刀菌及其专化型的快速检测与鉴别分子方法。
N Biotechnol. 2010 Sep 30;27(4):409-18. doi: 10.1016/j.nbt.2010.05.005. Epub 2010 May 13.
8
The causal agents of damping-off disease of buglosse from Iran.伊朗紫草猝倒病的致病因子。
Commun Agric Appl Biol Sci. 2005;70(3):319-22.
9
A newly developed real-time PCR assay for detection and quantification of Fusarium oxysporum and its use in compatible and incompatible interactions with grafted melon genotypes.一种新开发的实时 PCR 检测方法，用于检测和定量尖孢镰刀菌及其在与嫁接甜瓜基因型的亲和和非亲和互作中的应用。
Phytopathology. 2013 Aug;103(8):802-10. doi: 10.1094/PHYTO-11-12-0293-R.
10
Sphaerodes mycoparasitica sp. nov., a new biotrophic mycoparasite on Fusarium avenaceum, F. graminearum and F. oxysporum.球孢菌寄生霉新种（Sphaerodes mycoparasitica sp. nov.），一种寄生于燕麦镰孢菌、禾谷镰孢菌和尖孢镰孢菌的新的活体营养型真菌寄生菌。
Mycol Res. 2009 Oct;113(Pt 10):1172-80. doi: 10.1016/j.mycres.2009.07.018.

引用本文的文献

1
Colorimetry has practical advantages over spectrophotometry for estimating entomopathogenic fungi spore concentration in aqueous suspensions.在估计水悬浮液中昆虫病原真菌孢子浓度方面，比色法相对于分光光度法具有实际优势。
Folia Microbiol (Praha). 2025 Sep 2. doi: 10.1007/s12223-025-01320-1.
2
Exogenous Application of dsRNA-Inducing Silencing of the Gene and Reducing Its Virulence.dsRNA 诱导的基因沉默及其减毒作用的外源应用。
Int J Mol Sci. 2024 Sep 24;25(19):10286. doi: 10.3390/ijms251910286.
3
A New Benzaldehyde Derivative Exhibits Antiaflatoxigenic Activity against .
一种新型苯甲醛衍生物对……表现出抗黄曲霉毒素生成活性。
J Fungi (Basel). 2023 Nov 12;9(11):1103. doi: 10.3390/jof9111103.
4
Methods for Fungicide Efficacy Screenings: Multiwell Testing Procedures for the Oomycetes and .杀真菌剂药效筛选方法：卵菌纲的多孔板测试程序及…… （原文此处不完整）
Microorganisms. 2023 Jan 31;11(2):350. doi: 10.3390/microorganisms11020350.
5
Adjustment and quantification of UV-visible spectrophotometry analysis: an accurate and rapid method for estimating Cladosporium spp. spore concentration in a water suspension.紫外可见分光光度法分析的调整与定量：一种准确快速估算水悬浮液中枝孢菌属孢子浓度的方法。
World J Microbiol Biotechnol. 2022 Aug 12;38(10):183. doi: 10.1007/s11274-022-03356-8.
6
Biological Control of Celery Powdery Mildew Disease Caused by DC In Vitro and In Vivo Conditions.二孢白粉菌引起的芹菜白粉病在体外和体内条件下的生物防治
Plants (Basel). 2021 Oct 29;10(11):2342. doi: 10.3390/plants10112342.
7
Linking physiological parameters with visible/near-infrared leaf reflectance in the incubation period of vascular wilt disease.在维管束萎蔫病潜伏期将生理参数与可见/近红外叶片反射率相联系
Saudi J Biol Sci. 2020 Jan;27(1):88-99. doi: 10.1016/j.sjbs.2019.05.007. Epub 2019 May 31.
8
Evaluation of the Copan ESwab Transport System for Viability of Pathogenic Fungi by Use of a Modification of Clinical and Laboratory Standards Institute Document M40-A2.利用临床和实验室标准协会文件 M40-A2 的修订版评估 Copan ESwab 转运系统对致病性真菌的存活能力。
J Clin Microbiol. 2018 Jan 24;56(2). doi: 10.1128/JCM.01481-17. Print 2018 Feb.