Kopalli Spandana Rajendra, Cha Kyu-Min, Ryu Ji-Hoon, Lee Sang-Ho, Jeong Min-Sik, Hwang Seock-Yeon, Lee Young-Joo, Song Hee-Won, Kim Su-Nam, Kim Jin-Chul, Kim Si-Kwan
Department of Biomedical Chemistry, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Republic of Korea.
Department of Biomedical Laboratory Science, College of Applied Science and Industry, Daejeon University, Daejeon 300-716, Republic of Korea.
Exp Gerontol. 2017 Apr;90:26-33. doi: 10.1016/j.exger.2017.01.020. Epub 2017 Jan 23.
Korean red ginseng (Panax ginseng Meyer) is known to rejuvenate testicular effectiveness and the sperm maturation process by regulating redox proteins in aged rats. This study was performed to investigate the effect of Korean red ginseng water extract (KRG-WE) on the expression level of spermatogenesis-related key biomolecules and sex hormone receptors as well as enzymes regulating oxidation, histone deacetylation, and growth-related activities in aged rat testis. KRG-WE (200mg/kg) mixed with a regular pellet diet was administered to 12-month-old rats for 6months (KRG-AC), whereas the young (YC, 2months) and aged (AC, 12months) controls received the vehicle only. The results showed that the expression levels of spermatogenesis-related key biomolecules (inhibin-α, nectin-2, and cyclic adenosine monophosphate [cAMP] responsive element binding protein [CREB]-1), sex hormone receptors (androgen, luteinizing- and follicle-stimulating hormone receptors [AR, LHR, and FSHR, respectively]), and antioxidant enzymes (glutathione S-transferase mu [GSTm]-5, glutathione peroxidase [GPx]-4, peroxiredoxin [PRx]-3), as well as histone deactylation (silent mating type information regulation 2 homolog 1, SIRT1) and growth-related (mammalian target of rapamycin complex 1, mTORC1) molecules were significantly altered in the AC group rat testes compared with those of the YC group. However, KRG-WE treatment of the AC group significantly (p<0.05) attenuated these molecular changes. From these results, it can be concluded that long-term administration of KRG-WE significantly delayed the aging-induced testicular dysfunction.
众所周知，韩国红参（人参）可通过调节老龄大鼠体内的氧化还原蛋白来恢复睾丸功能及精子成熟过程。本研究旨在探讨韩国红参水提取物（KRG-WE）对老龄大鼠睾丸中精子发生相关关键生物分子、性激素受体以及调节氧化、组蛋白去乙酰化和生长相关活性的酶的表达水平的影响。将KRG-WE（200mg/kg）与常规颗粒饲料混合后给予12月龄大鼠，持续6个月（KRG-AC组），而年轻对照组（YC，2月龄）和老龄对照组（AC，12月龄）仅给予赋形剂。结果显示，与YC组相比，AC组大鼠睾丸中精子发生相关关键生物分子（抑制素-α、连接蛋白-2和环磷酸腺苷[cAMP]反应元件结合蛋白[CREB]-1）、性激素受体（雄激素、黄体生成素和促卵泡激素受体[分别为AR、LHR和FSHR]）以及抗氧化酶（谷胱甘肽S-转移酶μ[GSTm]-5、谷胱甘肽过氧化物酶[GPx]-4、过氧化物还原酶[PRx]-3）的表达水平，以及组蛋白去乙酰化（沉默交配型信息调节2同源物1，SIRT1）和生长相关（雷帕霉素靶蛋白复合物1，mTORC1）分子均发生了显著变化。然而，KRG-WE对AC组的治疗显著（p<0.05）减弱了这些分子变化。从这些结果可以得出结论，长期给予KRG-WE可显著延缓衰老诱导的睾丸功能障碍。
