Llena Carmen, Forner Leopoldo, Cambralla Raquel, Lozano Adrian
Department of Stomatology, Universitat de València, Valencia, Spain.
Private practice, Palma de Mallorca, Spain.
Restor Dent Endod. 2015 May;40(2):143-8. doi: 10.5395/rde.2015.40.2.143. Epub 2015 Feb 11.
This study evaluated the maximum depth and percentage of irrigant penetration into dentinal tubules by passive ultrasonic irrigation (PUI).
Thirty extracted human teeth were instrumented and divided into three groups. According to final irrigation regimen, 5.25% sodium hypochlorite (Group A, NaOCl), 2% chlorhexidine (Group B, CHX) and saline solution (Group C, control group) were applied with Irrisafe 20 tips (Acteon) and PUI. Irrigant was mixed with 0.1% rhodamine B. Sections at 2 mm, 5 mm, and 8 mm from the apex were examined with confocal laser scanning microscopy (CLSM). The percentage and maximum depth of irrigant penetration were measured. Kruskal-Wallis test and Mann-Whitney test were performed for overall comparison between groups at each level and for pairwise comparison, respectively. Within a group, Wilcoxon test was performed among different levels. p values less than 0.05 were considered significant.
In all groups, highest penetration depth and percentage of penetration were observed at the 8 mm level. At 2 mm level, Groups A and B had significantly greater depths and percentages in penetration than Group C (p < 0.05), but there were no significant differences between Groups A and B. At 5 mm level, penetration depths and percentage of penetration was not significantly different among the groups.
NaOCl and CHX applied by PUI showed similar depth and percentage of penetration at all evaluated levels.
本研究评估了被动超声冲洗（PUI）时冲洗剂渗入牙本质小管的最大深度和百分比。
选取30颗拔除的人类牙齿进行预备，分为三组。根据最终冲洗方案，使用Irrisafe 20冲洗头（Acteon公司）及PUI分别应用5.25%次氯酸钠（A组，NaOCl）、2%氯己定（B组，CHX）和生理盐水（C组，对照组）。冲洗剂与0.1%罗丹明B混合。用共聚焦激光扫描显微镜（CLSM）检查距根尖2mm、5mm和8mm处的切片。测量冲洗剂渗入的百分比和最大深度。分别采用Kruskal-Wallis检验和Mann-Whitney检验对各水平组间进行总体比较和两两比较。在同一组内，对不同水平进行Wilcoxon检验。p值小于0.05被认为具有统计学意义。
在所有组中，8mm水平处观察到最高的渗入深度和渗入百分比。在2mm水平处，A组和B组的渗入深度和百分比显著大于C组（p<0.05），但A组和B组之间无显著差异。在5mm水平处，各组间的渗入深度和渗入百分比无显著差异。
通过PUI应用的NaOCl和CHX在所有评估水平下显示出相似的渗入深度和百分比。
