癌症生物标志物在人血清中的长期稳定性：氧化应激和氧化还原状态的生物标志物、同型半胱氨酸、CRP以及谷丙转氨酶和谷氨酰转肽酶。
Long-term stability of cancer biomarkers in human serum: biomarkers of oxidative stress and redox status, homocysteine, CRP and the enzymes ALT and GGT.
作者信息
Jansen Eugène, Beekhof Piet, Viezeliene Dale, Muzakova Vladimira, Skalicky Jiri
机构信息
2Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, A Mickeviciaus 9, LT-44307, Kaunas, Lithuania.
出版信息
Biomark Med. 2015;9(5):425-32. doi: 10.2217/bmm.15.14.
AIM
Five frequently used biomarkers in cancer research and epidemiological studies were tested for their assay stability upon storage of serum for 12 months at -20 and -70/-80°C.
MATERIALS & METHODS: The biomarker assays include reactive oxygen metabolites (ROM), the total thiol levels (TTL), homocysteine (HCy), C-reactive protein (HS-CRP) and two liver enzymes, alanine aminotransferase (ALT) and γ-glutamyltransferase (GGT).
RESULTS
The assays for ROM, HCy, HS-CRP and GGT were stable in human serum samples at the two temperatures tested. The two other assays TTL and ALT, however, showed statistically significant differences in their stability between -20 and -80°C.
CONCLUSION
Therefore, storage at -80°C is advised to maintain a reliable assay outcome when serum samples have to be stored for longer periods.
目的
对癌症研究和流行病学研究中常用的五种生物标志物进行检测，以评估血清在-20℃和-70/-80℃下储存12个月后的检测稳定性。
材料与方法
生物标志物检测包括活性氧代谢产物（ROM）、总巯基水平（TTL）、同型半胱氨酸（HCy）、C反应蛋白（HS-CRP）以及两种肝酶，即丙氨酸转氨酶（ALT）和γ-谷氨酰转移酶（GGT）。
结果
在测试的两种温度下，ROM、HCy、HS-CRP和GGT的检测在人血清样本中是稳定的。然而，另外两种检测指标TTL和ALT在-20℃和-80℃之间的稳定性存在统计学显著差异。
结论
因此，当血清样本必须长期储存时，建议在-80℃下储存以维持可靠的检测结果。
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