Jansen Eugène, Beekhof Piet, Viezeliene Dale, Muzakova Vladimira, Skalicky Jiri
2Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, A Mickeviciaus 9, LT-44307, Kaunas, Lithuania.
Biomark Med. 2015;9(5):425-32. doi: 10.2217/bmm.15.14.
Five frequently used biomarkers in cancer research and epidemiological studies were tested for their assay stability upon storage of serum for 12 months at -20 and -70/-80°C.
MATERIALS & METHODS: The biomarker assays include reactive oxygen metabolites (ROM), the total thiol levels (TTL), homocysteine (HCy), C-reactive protein (HS-CRP) and two liver enzymes, alanine aminotransferase (ALT) and γ-glutamyltransferase (GGT).
The assays for ROM, HCy, HS-CRP and GGT were stable in human serum samples at the two temperatures tested. The two other assays TTL and ALT, however, showed statistically significant differences in their stability between -20 and -80°C.
Therefore, storage at -80°C is advised to maintain a reliable assay outcome when serum samples have to be stored for longer periods.
对癌症研究和流行病学研究中常用的五种生物标志物进行检测，以评估血清在-20℃和-70/-80℃下储存12个月后的检测稳定性。
生物标志物检测包括活性氧代谢产物（ROM）、总巯基水平（TTL）、同型半胱氨酸（HCy）、C反应蛋白（HS-CRP）以及两种肝酶，即丙氨酸转氨酶（ALT）和γ-谷氨酰转移酶（GGT）。
在测试的两种温度下，ROM、HCy、HS-CRP和GGT的检测在人血清样本中是稳定的。然而，另外两种检测指标TTL和ALT在-20℃和-80℃之间的稳定性存在统计学显著差异。
因此，当血清样本必须长期储存时，建议在-80℃下储存以维持可靠的检测结果。
