a Centre for Health Protection, National Institute of Public Health and the Environment , Bilthoven , the Netherlands.
b Department of Biochemistry , Lithuanian University of Health Sciences , Kaunas , Lithuania.
Free Radic Res. 2017 Dec;51(11-12):970-977. doi: 10.1080/10715762.2017.1398403.
The storage time and storage temperature might affect stability of oxidative stress biomarkers, therefore, they have to be analyzed after long-term storage of serum samples. The stability of three biomarkers reflecting oxidative stress: reactive oxygen metabolites (ROM) for hydroperoxides, total thiol levels (TTL) for the redox status and biological antioxidant potency (BAP) for the antioxidant status, was investigated at several time points during 60 months of storage at -20 and -80 °C. Biomarkers ROM and BAP showed a very good stability during storage for 60 months at both temperatures. In addition, the correlation of the data after 60 months of storage compared with the starting data was very good with correlation coefficients >0.9. The TTL assay showed good results in serum samples stored at -80 °C, but not in samples stored at -20 °C. Serum samples for analysis of the set of oxidative stress biomarkers ROM, BAP and TTL can be stored up to 60 months at -80 °C. ROM and BAP can also be stored at -20 °C during this period. The present results are very important for the biomarker-related epidemiological studies that make use of biobanks with samples stored for many years and for new project planning, including sample storage conditions.
储存时间和储存温度可能会影响氧化应激生物标志物的稳定性，因此，必须在血清样本长期储存后进行分析。本研究考察了在 -20℃和 -80℃下储存 60 个月过程中，三种反映氧化应激的生物标志物：活性氧代谢物（ROM）用于过氧化物，总巯基水平（TTL）用于氧化还原状态和生物抗氧化能力（BAP）用于抗氧化状态的稳定性。在这两种温度下，生物标志物 ROM 和 BAP 在 60 个月的储存期间表现出非常好的稳定性。此外，储存 60 个月后的数据与初始数据的相关性非常好，相关系数>0.9。TTL 测定在 -80℃储存的血清样本中显示出良好的结果，但在 -20℃储存的样本中则不然。用于分析氧化应激生物标志物 ROM、BAP 和 TTL 的一组血清样本可在 -80℃下储存长达 60 个月。在此期间，ROM 和 BAP 也可以在 -20℃下储存。这些结果对于利用储存多年的生物样本库进行生物标志物相关的流行病学研究以及新的项目规划非常重要，包括样本储存条件。
