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采用高效液相色谱-电喷雾串联质谱法鉴定巴西樱桃(Grumixama)中的鞣花单宁和类黄酮。

Identification of Ellagitannins and Flavonoids from Eugenia brasilienses Lam. (Grumixama) by HPLC-ESI-MS/MS.

机构信息

§Laboratory of Pharmacognosy, Experimental Station of Itajai, Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri), Rod. Antonio Heil, 6800, 88318-112 Itajaí, SC, Brazil.

出版信息

J Agric Food Chem. 2015 Jun 10;63(22):5417-27. doi: 10.1021/acs.jafc.5b01195. Epub 2015 Jun 2.

DOI:10.1021/acs.jafc.5b01195
PMID:25990484
Abstract

The grumixama (Eugenia brasiliensis Lam.), also known as Brazilian cherry, is a fruit native to Brazil. This study identified the flavonoids in the flesh and seeds and ellagitannin in the flesh of purple and yellow varieties. The physicochemical characteristics and antioxidant capacity of these fruits were also evaluated. Anthocyanins and flavonols were found in high levels in the flesh of purple (32-180 mg 100 g(-1) FW) and yellow grumixama (13-41 mg 100 g(-1) FW), respectively. The major flavonoids identified were cyanidin 3-glucoside and quercetin aglycone. Furthermore, ellagitannins were found in high levels in the flesh of purple (82-243 mg ellagic acid equiv 100 g(-1) FW) and yellow grumixama (92 mg ellagic acid equiv 100 g(-1) FW) and seeds (2220-2905 mg ellagic acid equiv 100 g(-1) FW). The ellagitannin profiles of both varieties were first characterized in which pedunculagin isomers, strictinin isomers, and ellagic acid galloyl hexoside were the major ellagitannins identified. In summary, both varieties of the grumixama fruit as well as the seeds could be good sources of bioactive compounds, mainly ellagitannins.

摘要

嘉宝果(Eugenia brasiliensis Lam.),又称巴西樱桃,原产于巴西。本研究鉴定了其果肉和种子中的类黄酮和果肉中的鞣花单宁。还评估了这些水果的理化特性和抗氧化能力。在紫色(32-180 mg 100 g(-1)FW)和黄色嘉宝果(13-41 mg 100 g(-1)FW)的果肉中发现了高浓度的花青素和类黄酮,分别。鉴定出的主要类黄酮为矢车菊素 3-葡萄糖苷和槲皮素糖苷。此外,在紫色(82-243 mg 鞣花酸当量 100 g(-1)FW)和黄色嘉宝果(92 mg 鞣花酸当量 100 g(-1)FW)和种子(2220-2905 mg 鞣花酸当量 100 g(-1)FW)的果肉中发现了高浓度的鞣花单宁。这两种品种的鞣花单宁谱是首次被鉴定出来的,其中发现了栓皮素异构体、苛辣霉素异构体和鞣花酸酰基葡萄糖苷是主要的鞣花单宁。总之,嘉宝果的果肉和种子都可以作为生物活性化合物的良好来源,主要是鞣花单宁。

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