McParland Aidan L, Follansbee Taylor L, Ganter Geoffrey K
Department of Biology, University of New England.
Department of Biology, University of New England;
J Vis Exp. 2015 Apr 30(98):e52684. doi: 10.3791/52684.
Drosophila larvae are used in many behavioral studies, yet a simple device for measuring basic parameters of larval activity has not been available. This protocol repurposes an instrument often used to measure adult activity, the TriKinetics Drosophila activity monitor (MB5 Multi-Beam Activity Monitor) to study larval activity. The instrument can monitor the movements of animals in 16 individual 8 cm glass assay tubes, using 17 infrared detection beams per tube. Logging software automatically saves data to a computer, recording parameters such as number of moves, times sensors were triggered, and animals' positions within the tubes. The data can then be analyzed to represent overall locomotion and/or position preference as well as other measurements. All data are easily accessible and compatible with basic graphing and data manipulation software. This protocol will discuss how to use the apparatus, how to operate the software and how to run a larval activity assay from start to finish.
果蝇幼虫被用于许多行为研究中，但一直没有一种简单的装置可用于测量幼虫活动的基本参数。本方案将一种常用于测量成虫活动的仪器——TriKinetics果蝇活动监测仪（MB5多光束活动监测仪）进行重新利用，以研究幼虫活动。该仪器可以使用每管17个红外检测光束，监测16个单独的8厘米玻璃测定管中动物的运动。记录软件会自动将数据保存到计算机中，记录移动次数、传感器触发次数以及动物在管内的位置等参数。然后可以对数据进行分析，以表示整体运动和/或位置偏好以及其他测量结果。所有数据都易于获取，并且与基本的绘图和数据处理软件兼容。本方案将讨论如何使用该仪器、如何操作软件以及如何从头到尾进行幼虫活动测定。
