病毒学。一种感染嗜热菌的病毒包裹着A型DNA。
Virology. A virus that infects a hyperthermophile encapsidates A-form DNA.
作者信息
DiMaio Frank, Yu Xiong, Rensen Elena, Krupovic Mart, Prangishvili David, Egelman Edward H
机构信息
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA.
出版信息
Science. 2015 May 22;348(6237):914-7. doi: 10.1126/science.aaa4181.
Extremophiles, microorganisms thriving in extreme environmental conditions, must have proteins and nucleic acids that are stable at extremes of temperature and pH. The nonenveloped, rod-shaped virus SIRV2 (Sulfolobus islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, which lives at 80°C and pH 3. We have used cryo-electron microscopy to generate a three-dimensional reconstruction of the SIRV2 virion at ~4 angstrom resolution, which revealed a previously unknown form of virion organization. Although almost half of the capsid protein is unstructured in solution, this unstructured region folds in the virion into a single extended α helix that wraps around the DNA. The DNA is entirely in the A-form, which suggests a common mechanism with bacterial spores for protecting DNA in the most adverse environments.
嗜极生物是在极端环境条件下繁衍生息的微生物，它们必须拥有在极端温度和pH值下仍保持稳定的蛋白质和核酸。无包膜的杆状病毒SIRV2（冰岛硫化叶菌杆状病毒2）感染嗜热嗜酸的冰岛硫化叶菌，该菌生活在80°C和pH值为3的环境中。我们利用冷冻电子显微镜以约4埃的分辨率生成了SIRV2病毒粒子的三维重建图，揭示了一种此前未知的病毒粒子组织形式。尽管几乎一半的衣壳蛋白在溶液中是无结构的，但这个无结构区域在病毒粒子中折叠成一个单一的延伸α螺旋，环绕着DNA。DNA完全呈A形态，这表明它与细菌孢子在最恶劣环境中保护DNA有着共同的机制。
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