胃饥饿素对大鼠睾丸缺血再灌注中生殖细胞凋亡及促炎细胞因子产生的影响。
Effects of Ghrelin on germ cell apoptosis and proinflammatory cytokines production in Ischemia-reperfusion of the rat testis.
作者信息
Taati Majid, Moghadasi Mehrnoush, Dezfoulian Omid, Asadian Payman, Zendehdel Morteza
机构信息
Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
Department of Physiology, Faculty of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran.
出版信息
Iran J Reprod Med. 2015 Feb;13(2):85-92.
BACKGROUND
Testicular torsion is a medical emergency that requires surgical intervention to reperfuse the affected testis. Ischemia reperfusion injury is usually associated with proinflammatory cytokine generation and apoptosis of germ cells in the testes.
OBJECTIVE
In this study we investigate the effect of ghrelin on the proinflammatory cytokines levels and germ cell apoptosis in testicular ischemia reperfusion.
MATERIALS AND METHODS
45 male rats were selected for the study and randomly divided into 3 groups, each containing 15 rats. Animals in the testicular torsion and ghrelin treated groups were subjected to unilateral 720 counterclockwise testicular torsion for 1 hr and then reperfusion was allowed after detorsion for 4 hr, 1 and 7 days. The ghrelin-treated group received intraperitoneal injection of ghrelin 15min before detorsion. The expression levels of bcl-2-associated X protein and proliferating cell nuclear antigen in testicular tissue in the different groups were detected by immunohistochemical assay and tissue cytokines interleukin-1β, tumor necroses factor-α and interleukin-6 were measured using enzyme-linked immunosorbent assay.
RESULTS
After being treated by ghrelin, the population of immunoreactive cells against BAX in the spermatocytes on day 7 after reperfusion significantly decreased when compared to tortion/ detortion-saline animals (p=0.024). In contrast, PCNA expression in the spermatocytes and spermatogonia were not significantly different between tortion/ detortion-ghrelin and tortion/ detortion-saline groups on both experimental days. Administration of ghrelin significantly attenuated the testicular tumor necroses factor-α and interleukin-6 levels compared with the untreated animals, but had no significant effect on the level of interleukin-1β.
CONCLUSION
Ghrelin offers remarkable anti-inflammatory and anti-apoptotic effects in testicular ischemia reperfusion injury.
背景
睾丸扭转是一种需要手术干预以使受影响睾丸重新灌注的医疗急症。缺血再灌注损伤通常与睾丸中促炎细胞因子的产生以及生殖细胞的凋亡有关。
目的
在本研究中，我们调查了胃饥饿素对睾丸缺血再灌注中促炎细胞因子水平和生殖细胞凋亡的影响。
材料与方法
选择45只雄性大鼠进行研究，并随机分为3组，每组15只。睾丸扭转和胃饥饿素治疗组的动物进行单侧逆时针720度睾丸扭转1小时，然后在扭转解除后分别再灌注4小时、1天和7天。胃饥饿素治疗组在扭转解除前15分钟腹腔注射胃饥饿素。通过免疫组织化学测定法检测不同组睾丸组织中bcl-2相关X蛋白和增殖细胞核抗原的表达水平，并使用酶联免疫吸附测定法测量组织细胞因子白细胞介素-1β、肿瘤坏死因子-α和白细胞介素-6。
结果
与扭转/解除扭转-生理盐水处理的动物相比，胃饥饿素处理后，再灌注7天后精母细胞中抗BAX免疫反应性细胞数量显著减少（p=0.024）。相比之下，在两个实验日，扭转/解除扭转-胃饥饿素组和扭转/解除扭转-生理盐水组的精母细胞和精原细胞中PCNA表达没有显著差异。与未处理的动物相比，胃饥饿素给药显著降低了睾丸肿瘤坏死因子-α和白细胞介素-6水平，但对白细胞介素-1β水平没有显著影响。
结论
胃饥饿素在睾丸缺血再灌注损伤中具有显著的抗炎和抗凋亡作用。
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