Ninomiya Y, Sako N, Funakoshi M
Department of Oral Physiology, Asahi University School of Dentistry, Gifu, Japan.
J Comp Physiol A. 1989 Nov;166(1):1-5. doi: 10.1007/BF00190204.
The effects of lingual treatment with amiloride, an inhibitor of salt taste responses in several mammalian species, on NaCl responses of the chorda tympani nerve were compared between four inbred strains of mouse (BALB/cCrSlc, DBA/2CrSlc, C57BL/6CrSlc and C3H/HeSlc). In C57BL and C3H mice amiloride significantly suppressed responses of the chorda tympani nerve to NaCl at a concentration 0.1 M or more whereas in BALB and DBA mice the drug did not significantly affect the responses to NaCl at any concentration, suggesting a lack of the amiloride-sensitive receptor component for NaCl in the latter two strains. A two-bottle preference test demonstrated that all strains of mouse usually showed no preference for NaCl at any concentration and avoided NaCl at 0.3 M or more, although some differences were observed in that C57BL and C3H mice showed aversive responses to 0.1 and 0.15 M NaCl, whereas BALB and DBA mice were indifferent to these solutions. The results suggest that there exist prominent differences between mouse strains in the amiloride-sensitive component of their salt receptor systems. However, in mice the taste information derived from the amiloride-sensitive receptor component probably has no remarkable effect on behavioral responses to NaCl except for a possible contribution to decreasing aversion thresholds for NaCl by increasing overall taste information about NaCl.
在四种近交系小鼠（BALB/cCrSlc、DBA/2CrSlc、C57BL/6CrSlc和C3H/HeSlc）中，比较了用阿米洛利（几种哺乳动物盐味反应的抑制剂）进行舌部处理对鼓索神经NaCl反应的影响。在C57BL和C3H小鼠中，阿米洛利在浓度为0.1 M或更高时显著抑制鼓索神经对NaCl的反应，而在BALB和DBA小鼠中，该药物在任何浓度下均未显著影响对NaCl的反应，这表明后两个品系中缺乏对NaCl敏感的阿米洛利受体成分。双瓶偏好试验表明，所有品系的小鼠通常对任何浓度的NaCl都没有偏好，并且在0.3 M或更高浓度时会避开NaCl，尽管观察到一些差异，即C57BL和C3H小鼠对0.1和0.15 M NaCl表现出厌恶反应，而BALB和DBA小鼠对这些溶液无动于衷。结果表明，小鼠品系之间的盐受体系统的阿米洛利敏感成分存在显著差异。然而，在小鼠中，来自阿米洛利敏感受体成分的味觉信息可能对NaCl的行为反应没有显著影响，除了可能通过增加关于NaCl的整体味觉信息来降低对NaCl的厌恶阈值。
