Bodor Peter, Szoke Antal, Toth-Lencses Kitti, Veres Aniko, Deak Tamas, Kozma Pal, Bisztray Gyorgy Denes, Kiss Erzsebet
Institute of Viticulture and Enology, Corvinus University of Budapest , Budapest , Hungary.
Institute of Genetics and Biotechnology, Szent Istvan University , Gödöllő , Hungary.
Biotechnol Biotechnol Equip. 2014 Jan 2;28(1):14-20. doi: 10.1080/13102818.2014.901666.
Twenty-seven grapevine () varieties within 12 putative berry colour variation groups () were genotyped with 14 highly polymorphic microsatellite (simple sequence repeats (SSR)) markers. Three additional oligonucleotide primers were applied for the detection of the Gret1 retroelement insertion in the promoter region of transcription factor gene regulating the UFGT (UDP-glucose: flavonoid 3-O-glucosyltransferase) activity. UFGT is the key enzyme of the anthocyanin biosynthetic pathway. SSR results proved that the analysed cultivars can be grouped only into nine , the other three putative berry colour variant groups consist of homonyms as a consequence of misnaming. In the case of Sárfehér-Sárpiros, Delaware red-Delaware white and Járdovány fekete-Járdovány fehér, it was attested that they are not bud sports, but homonyms. Some members could be differentiated according to the presence or the absence of the retroelement (Chasselas, Furmint and Lisztes), while others, Bajor, Bakator, Gohér and Traminer members, remained indistinguishable either by the microsatellites or the -based method.
利用14个高度多态性微卫星（简单序列重复，SSR）标记对12个假定的浆果颜色变异组中的27个葡萄品种进行了基因分型。另外使用了3种寡核苷酸引物来检测调控UDP-葡萄糖：类黄酮3-O-葡萄糖基转移酶（UFGT）活性的转录因子基因启动子区域中Gret1反转录元件的插入情况。UFGT是花青素生物合成途径的关键酶。SSR结果表明，所分析的品种只能分为9个组，另外3个假定的浆果颜色变异组由于命名错误而由同名品种组成。对于Sárfehér-Sárpiros、特拉华红-特拉华白和Járdovány fekete-Járdovány fehér，已证实它们不是芽变品种，而是同名品种。一些组的成员可以根据反转录元件的存在与否进行区分（霞多丽、福尔明特和利斯特斯），而其他组，如Bajor、Bakator、Gohér和Traminer组的成员，无论是通过微卫星还是基于反转录元件的方法都无法区分。
