Pejchar Přemysl, Martinec Jan
a Institute of Experimental Botany, v. v. i.; Academy of Sciences of the Czech Republic ; Prague , Czech Republic.
Plant Signal Behav. 2015;10(6):e1031938. doi: 10.1080/15592324.2015.1031938.
The first indication of the aluminum (Al) toxicity in plants growing in acidic soils is the cessation of root growth, but the detailed mechanism of Al effect is unknown. Here we examined the impact of Al stress on the activity of non-specific phospholipase C (NPC) in the connection with the processes related to the plasma membrane using fluorescently labeled phosphatidylcholine. We observed a rapid and significant decrease of labeled diacylglycerol (DAG), product of NPC activity, in Arabidopsis seedlings treated with AlCl₃. Interestingly, an application of the membrane fluidizer, benzyl alcohol, restored the level of DAG during Al treatment. Our observations suggest that the activity of NPC is affected by Al-induced changes in plasma membrane physical properties.
在酸性土壤中生长的植物，铝（Al）毒性的首个迹象是根系生长停止，但铝作用的详细机制尚不清楚。在这里，我们使用荧光标记的磷脂酰胆碱，研究了铝胁迫对非特异性磷脂酶C（NPC）活性的影响，以及与质膜相关过程的联系。我们观察到，用AlCl₃处理的拟南芥幼苗中，NPC活性产物标记的二酰基甘油（DAG）迅速且显著减少。有趣的是，施用膜流化剂苯甲醇可在铝处理期间恢复DAG水平。我们的观察结果表明，NPC的活性受铝诱导的质膜物理性质变化的影响。
