Wei Xue-Qin, Hao Li-Ying, Shao Xiao-Ru, Zhang Quan, Jia Xiao-Qin, Zhang Zhi-Rong, Lin Yun-Feng, Peng Qiang
†State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
‡Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
ACS Appl Mater Interfaces. 2015 Jun 24;7(24):13367-74. doi: 10.1021/acsami.5b01874. Epub 2015 Jun 10.
As novel applied nanomaterials, both graphene oxide (GO) and its reduced form (rGO) have attracted global attention, because of their excellent properties. However, the lack of comprehensive understanding of their interactions with biomacromolecules highly limits their biomedical applications. This work aims to initiate a systematic study on the property changes of GO/rGO upon interaction with serum proteins and on how their degree of reduction and exposure concentration affect this interaction, as well as to analyze the possible biomedical impacts of the interaction. We found that the adsorption of proteins on GO/rGO occurred spontaneously and rapidly, leading to significant changes in size, zeta potential, and morphology. Compared to rGO, GO showed a higher ability in quenching intrinsic fluorescence of serum proteins in a concentration-dependent manner. The protein adsorption efficiency and the types of associated proteins varied, depending on the degree of reduction and concentration of graphene. Our findings indicate the importance of evaluating the potential protein adsorption before making use of GO/rGO in drug delivery, because the changed physicochemical properties after protein adsorption will have significant impacts on safety and effectiveness of these delivery systems. On the other hand, this interaction can also be used for the separation, purification, or delivery of certain proteins.
作为新型应用纳米材料，氧化石墨烯（GO）及其还原形式（rGO）因其优异性能而受到全球关注。然而，对它们与生物大分子相互作用缺乏全面了解，这极大地限制了它们在生物医学领域的应用。这项工作旨在对GO/rGO与血清蛋白相互作用时的性质变化、还原程度和暴露浓度如何影响这种相互作用展开系统研究，并分析这种相互作用可能产生的生物医学影响。我们发现，蛋白质在GO/rGO上的吸附是自发且快速发生的，导致尺寸、zeta电位和形态发生显著变化。与rGO相比，GO在以浓度依赖方式淬灭血清蛋白固有荧光方面表现出更高的能力。蛋白质吸附效率和相关蛋白质类型因石墨烯的还原程度和浓度而异。我们的研究结果表明，在将GO/rGO用于药物递送之前评估潜在蛋白质吸附的重要性，因为蛋白质吸附后物理化学性质的改变将对这些递送系统的安全性和有效性产生重大影响。另一方面，这种相互作用也可用于某些蛋白质的分离、纯化或递送。
