Carpenter Bradley L, Situ Xingci, Scholle Frank, Bartelmess Juergen, Weare Walter W, Ghiladi Reza A
Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA.
Department of Biological Sciences, North Carolina State University, Raleigh, NC 27695-7614, USA.
Molecules. 2015 Jun 8;20(6):10604-21. doi: 10.3390/molecules200610604.
Antimicrobial photodynamic inactivation (aPDI) employing the BODIPY-based photosensitizer 2,6-diiodo-1,3,5,7-tetramethyl-8-(N-methyl-4-pyridyl)-4,4'-difluoro-boradiazaindacene (DIMPy-BODIPY) was explored in an in vitro assay against six species of bacteria (eight total strains), three species of yeast, and three viruses as a complementary approach to their current drug-based or non-existent treatments. Our best results achieved a noteworthy 5-6 log unit reduction in CFU at 0.1 μM for Staphylococcus aureus (ATCC-2913), methicillin-resistant S. aureus (ATCC-44), and vancomycin-resistant Enterococcus faecium (ATCC-2320), a 4-5 log unit reduction for Acinetobacter baumannii ATCC-19606 (0.25 μM), multidrug resistant A. baumannii ATCC-1605 (0.1 μM), Pseudomonas aeruginosa ATCC-97 (0.5 μM), and Klebsiella pneumoniae ATCC-2146 (1 μM), and a 3 log unit reduction for Mycobacterium smegmatis mc2155 (ATCC-700084). A 5 log unit reduction in CFU was observed for Candida albicans ATCC-90028 (1 μM) and Cryptococcus neoformans ATCC-64538 (0.5 μM), and a 3 log unit reduction was noted for Candida glabrata ATCC-15545 (1 μM). Infectivity was reduced by 6 log units in dengue 1 (0.1 μM), by 5 log units (0.5 μM) in vesicular stomatitis virus, and by 2 log units (5 μM) in human adenovirus-5. Overall, the results demonstrate that DIMPy-BODIPY exhibits antiviral, antibacterial and antifungal photodynamic inactivation at nanomolar concentrations and short illumination times.
采用基于BODIPY的光敏剂2,6-二碘-1,3,5,7-四甲基-8-(N-甲基-4-吡啶基)-4,4'-二氟硼二氮杂萘（DIMPy-BODIPY）的抗菌光动力灭活（aPDI）在体外针对六种细菌（共八个菌株）、三种酵母和三种病毒进行了研究，作为其当前基于药物或不存在治疗方法的补充手段。我们的最佳结果显示，对于金黄色葡萄球菌（ATCC-2913）、耐甲氧西林金黄色葡萄球菌（ATCC-44）和耐万古霉素粪肠球菌（ATCC-2320），在0.1 μM时CFU显著降低5-6个对数单位；对于鲍曼不动杆菌ATCC-19606（0.25 μM）、多重耐药鲍曼不动杆菌ATCC-1605（0.1 μM）、铜绿假单胞菌ATCC-97（0.5 μM）和肺炎克雷伯菌ATCC-2146（1 μM），CFU降低4-5个对数单位；对于耻垢分枝杆菌mc2155（ATCC-700084），CFU降低3个对数单位。对于白色念珠菌ATCC-90028（1 μM）和新型隐球菌ATCC-64538（0.5 μM），观察到CFU降低5个对数单位，对于光滑念珠菌ATCC-15545（1 μM），CFU降低3个对数单位。登革热1型（0.1 μM）的感染性降低6个对数单位，水疱性口炎病毒（0.5 μM）降低5个对数单位，人腺病毒-5（5 μM）降低2个对数单位。总体而言，结果表明DIMPy-BODIPY在纳摩尔浓度和短光照时间下表现出抗病毒、抗菌和抗真菌的光动力灭活作用。
