Shen Xiao-Min, Zhong Rui, Xia Wen-Kai, Wei Dong, Ding Tian-Bo, Liao Chong-Yu, Niu Jin-Zhi, Dou Wei, Wang Jin-Jun
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; Zhaotong City Branch, Yunnan Provincial Tobacco Corporation, Yunnan 657000, China.
J Proteomics. 2017 Mar 31;158:9-19. doi: 10.1016/j.jprot.2017.01.020. Epub 2017 Feb 20.
Abamectin is a microbial-derived pesticide widely used for control of agricultural pests. However, sustained use of abamectin has led to the development of resistance in some target species. Previous studies on arthropod resistance to abamectin have mainly used traditional biochemical and molecular approaches. To understand the responses of citrus red mite, Panonychus citri, exposed to abamectin, comparative proteomic analysis was conducted using two-dimensional electrophoresis (2-DE). A total of 26 distinct protein spots were present in response to abamectin exposure. Tandem mass spectrometry (MS/MS) identified 16 proteins that were mainly involved in energy metabolism and detoxification. Some remaining proteins were not identifiable, suggesting that they may be novel. The expression levels of transcripts associated with proteins were analyzed by quantitative reverse transcription PCR (qRT-PCR). Furthermore, to validate the proteomic data obtained in the present study, Western-blot experiment was performed and the expression of sHsp and PcE1 proteins were confirmed, respectively.
The citrus red mite has developed resistance to many acaricides, including abamectin. In the current study, we used the proteomic approaches involving 2-DE, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), and MS/MS to document changes in adult P. citri during 24h of abamectin exposure. Abamectin stress induced a total of 16 differentially regulated proteins. The proteomic results were validated in mRNA expression patterns using qRT-PCR. This is the first analysis of differentially expressed proteins in P. citri exposed to abamectin. The results help clarify the physiological mechanisms of P. citri responses to abamectin exposure.
阿维菌素是一种微生物源农药，广泛用于防治农业害虫。然而，持续使用阿维菌素已导致一些靶标物种产生抗性。以往关于节肢动物对阿维菌素抗性的研究主要采用传统的生化和分子方法。为了解柑橘全爪螨（Panonychus citri）暴露于阿维菌素后的反应，使用二维电泳（2-DE）进行了比较蛋白质组学分析。暴露于阿维菌素后共出现26个不同的蛋白质斑点。串联质谱（MS/MS）鉴定出16种主要参与能量代谢和解毒的蛋白质。其余一些蛋白质无法鉴定，表明它们可能是新的蛋白质。通过定量逆转录PCR（qRT-PCR）分析了与蛋白质相关的转录本的表达水平。此外，为了验证本研究中获得的蛋白质组学数据，进行了蛋白质免疫印迹实验，分别证实了小分子热激蛋白（sHsp）和羧酸酯酶1（PcE1）蛋白的表达。
柑橘全爪螨已对包括阿维菌素在内的许多杀螨剂产生抗性。在本研究中，我们使用了涉及二维电泳、基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF）和串联质谱的蛋白质组学方法，记录柑橘全爪螨成虫在暴露于阿维菌素24小时期间的变化。阿维菌素胁迫共诱导了16种差异调节蛋白。使用qRT-PCR在mRNA表达模式中验证了蛋白质组学结果。这是首次对暴露于阿维菌素的柑橘全爪螨中差异表达蛋白的分析。结果有助于阐明柑橘全爪螨对阿维菌素暴露反应的生理机制。
