Karthikeyan Krishnamoorthy, Babu Anish, Kim Sang-Jae, Murugesan Ramachandran, Jeyasubramanian Kadarkaraithangam
Nanomaterials and System Laboratory, Department of Mechanical Engineering, Jeju National University, Jeju, South Korea ; Department of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi, Tamilnadu India.
School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamilnadu India.
Cancer Nanotechnol. 2011;2(1-6):95-103. doi: 10.1007/s12645-011-0019-3. Epub 2011 Aug 13.
Photodynamic therapy (PDT) is a promising treatment methodology whereby diseased cells and tissues are destroyed by reactive oxygen species (ROS) by using a combination of light and photosensitizers (PS). The medical application of Rose Bengal (RB), photosensitizer with very good ROS generation capability, is limited due to its intrinsic toxicity and insufficient lipophilicity. In this report, we evaluate the potential of polyamidoamine (PAMAM) dendrimers in delivering RB and its phototoxic efficiency towards a model cancer cell line. The spherical, nanoscaled dendrimers could efficiently encapsulate RB and showed characteristic spectral responses. The controlled release property of dendrimer-RB formulation was clearly evident from the in vitro drug release study. ROS generation was confirmed in dendrimer-RB system upon white light illumination. Photosensitization of Dalton's Lymphoma Ascite (DLA) cells incubated with dendrimer-RB formulation caused remarkable photocytotoxicity. Importantly, the use of dendrimer-based delivery system reduced the dark toxicity of RB.
光动力疗法（PDT）是一种很有前景的治疗方法，通过结合光和光敏剂（PS），利用活性氧（ROS）来破坏病变细胞和组织。孟加拉玫瑰红（RB）作为一种具有很强ROS生成能力的光敏剂，由于其内在毒性和脂溶性不足，其医学应用受到限制。在本报告中，我们评估了聚酰胺胺（PAMAM）树枝状大分子递送RB的潜力及其对模型癌细胞系的光毒性效率。球形的纳米级树枝状大分子能够有效地包裹RB，并表现出特征光谱响应。从体外药物释放研究中可以明显看出树枝状大分子-RB制剂的控释特性。白光照射后，树枝状大分子-RB体系中ROS生成得到证实。用树枝状大分子-RB制剂孵育的道尔顿淋巴瘤腹水（DLA）细胞的光致敏引起了显著的光细胞毒性。重要的是，基于树枝状大分子的递送系统的使用降低了RB的暗毒性。
