Larrañaga Aitor, Alonso-Varona Ana, Palomares Teodoro, Rubio-Azpeitia Eva, Aldazabal Pablo, Martin Francisco Javier, Sarasua Jose-Ramon
Department of Mining-Metallurgy Engineering and Materials Science & POLYMAT, University of the Basque Country (UPV/EHU), School of Engineering, Alameda de Urquijo s/n, 480130, Bilbao, Spain.
Faculty of Medicine and Odontology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
J Biomed Mater Res A. 2015 Dec;103(12):3815-24. doi: 10.1002/jbm.a.35525. Epub 2015 Jul 1.
Incorporation of bioactive glass (BG) particles to synthetic polymer scaffolds is a promising strategy to improve the bioactivity of bioinert materials and to stimulate specific cell responses. In this study, the influence of incorporating BG particles to lactide and caprolactone based porous scaffolds on osteogenic differentiation of adipose-derived stem cells (ASCs) was analyzed. Accordingly, ASCs were seeded on poly(L-lactide) (PLLA), poly(ε-caprolactone) (PCL), or poly(L-lactide-co-ε-caprolactone) (PLCL) scaffolds containing 15 vol % of BG particles in two culture conditions: standard versus osteogenic culture medium. In standard culture medium, incorporation of BG to a PLLA scaffold increased the ALP activity with respect to its unfilled counterpart (ca. 1.2- and a 1.6-fold increase over 7 and 14 days, respectively). Moreover, in all the studied polymers the incorporation of BG induced a slightly higher production of mineralized matrix by ASCs, but the differences observed were not statistically significant. In the osteogenic medium, the effect of BG was masked by the effect of osteogenic supplements in the long-term. However, in the short-term (day 7), BG particles induced an early ALP activity of predifferentiated osteoblasts on PLLA and PCL scaffolds and higher matrix mineralization on PCL scaffolds. In summary, the addition of BG particles to PLLA and PCL scaffolds sustains ASC osteogenic differentiation, facilitates mineralization and induces the formation of a hydroxyapatite layer on the surface of the polymer scaffolds.
将生物活性玻璃（BG）颗粒掺入合成聚合物支架是一种很有前景的策略，可提高生物惰性材料的生物活性并刺激特定的细胞反应。在本研究中，分析了将BG颗粒掺入基于丙交酯和己内酯的多孔支架对脂肪干细胞（ASC）成骨分化的影响。因此，将ASC接种在含有15体积%BG颗粒的聚（L-丙交酯）（PLLA）、聚（ε-己内酯）（PCL）或聚（L-丙交酯-co-ε-己内酯）（PLCL）支架上，置于两种培养条件下：标准培养基与成骨培养基。在标准培养基中，相对于未填充BG的PLLA支架，掺入BG可提高碱性磷酸酶（ALP）活性（在7天和14天时分别约增加1.2倍和1.6倍）。此外，在所有研究的聚合物中，掺入BG会使ASC产生的矿化基质略有增加，但观察到的差异无统计学意义。在成骨培养基中，从长期来看，BG的作用被成骨补充剂的作用所掩盖。然而，在短期内（第7天），BG颗粒可诱导预分化成骨细胞在PLLA和PCL支架上早期出现ALP活性，并使PCL支架上的基质矿化程度更高。总之，向PLLA和PCL支架中添加BG颗粒可维持ASC的成骨分化，促进矿化，并在聚合物支架表面诱导形成羟基磷灰石层。
