Rath Subha N, Nooeaid Patcharakamon, Arkudas Andreas, Beier Justus P, Strobel Leonie A, Brandl Andreas, Roether Judith A, Horch Raymund E, Boccaccini Aldo R, Kneser Ulrich
Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen-Nürnberg, Erlangen, Germany.
Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad, India.
J Tissue Eng Regen Med. 2016 Oct;10(10):E497-E509. doi: 10.1002/term.1849. Epub 2013 Dec 3.
Mesenchymal stem cells can be isolated from a variety of different sources, each having their own peculiar merits and drawbacks. Although a number of studies have been conducted comparing these stem cells for their osteo-differentiation ability, these are mostly done in culture plastics. We have selected stem cells from either adipose tissue (ADSCs) or bone marrow (BMSCs) and studied their differentiation ability in highly porous three-dimensional (3D) 45S5 Bioglass®-based scaffolds. Equal numbers of cells were seeded onto 5 × 5 × 4 mm scaffolds and cultured in vitro, with or without osteo-induction medium. After 2 and 4 weeks, the cell-scaffold constructs were analysed for cell number, cell spreading, viability, alkaline phosphatase activity and osteogenic gene expression. The scaffolds with ADSCs displayed osteo-differentiation even without osteo-induction medium; however, with osteo-induction medium osteogenic differentiation was further increased. In contrast, the scaffolds with BMSCs showed no osteo-differentiation without osteo-induction medium; after application of osteo-induction medium, osteo-differentiation was confirmed, although lower than in scaffolds with ADSCs. In general, stem cells in 3D bioactive glass scaffolds differentiated better than cells in culture plastics with respect to their ALP content and osteogenic gene expression. In summary, 45S5 Bioglass-based scaffolds seeded with ADSCs are well-suited for possible bone tissue-engineering applications. Induction of osteogenic differentiation appears unnecessary prior to implantation in this specific setting. Copyright © 2013 John Wiley & Sons, Ltd.
间充质干细胞可以从多种不同来源分离得到，每种来源都有其独特的优缺点。尽管已经进行了许多研究来比较这些干细胞的骨分化能力，但这些研究大多是在培养塑料中进行的。我们从脂肪组织（ADSCs）或骨髓（BMSCs）中选取干细胞，并研究它们在基于45S5生物活性玻璃®的高度多孔三维（3D）支架中的分化能力。将等量的细胞接种到5×5×4毫米的支架上，在有或没有骨诱导培养基的情况下进行体外培养。在2周和4周后，对细胞-支架构建体进行细胞数量、细胞铺展、活力、碱性磷酸酶活性和成骨基因表达的分析。接种ADSCs的支架即使在没有骨诱导培养基的情况下也显示出骨分化；然而，在添加骨诱导培养基后，成骨分化进一步增强。相比之下，接种BMSCs的支架在没有骨诱导培养基的情况下没有显示出骨分化；在应用骨诱导培养基后，虽然低于接种ADSCs的支架，但证实了骨分化。总体而言，在3D生物活性玻璃支架中的干细胞在碱性磷酸酶含量和成骨基因表达方面比在培养塑料中的细胞分化得更好。总之，接种ADSCs的基于45S5生物活性玻璃的支架非常适合可能的骨组织工程应用。在这种特定情况下，植入前似乎不需要诱导成骨分化。版权所有© 2013约翰威立父子有限公司。
