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WRKY33对脱落酸信号的负调控对拟南芥抵抗灰葡萄孢2100的免疫反应至关重要。

Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100.

作者信息

Liu Shouan, Kracher Barbara, Ziegler Jörg, Birkenbihl Rainer P, Somssich Imre E

机构信息

Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Köln, Germany.

Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Halle, Germany.

出版信息

Elife. 2015 Jun 15;4:e07295. doi: 10.7554/eLife.07295.

DOI:10.7554/eLife.07295
PMID:26076231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4487144/
Abstract

The Arabidopsis mutant wrky33 is highly susceptible to Botrytis cinerea. We identified >1680 Botrytis-induced WRKY33 binding sites associated with 1576 Arabidopsis genes. Transcriptional profiling defined 318 functional direct target genes at 14 hr post inoculation. Comparative analyses revealed that WRKY33 possesses dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. We confirmed known WRKY33 targets involved in hormone signaling and phytoalexin biosynthesis, but also uncovered a novel negative role of abscisic acid (ABA) in resistance towards B. cinerea 2100. The ABA biosynthesis genes NCED3 and NCED5 were identified as direct targets required for WRKY33-mediated resistance. Loss-of-WRKY33 function resulted in elevated ABA levels and genetic studies confirmed that WRKY33 acts upstream of NCED3/NCED5 to negatively regulate ABA biosynthesis. This study provides the first detailed view of the genome-wide contribution of a specific plant transcription factor in modulating the transcriptional network associated with plant immunity.

摘要

拟南芥突变体wrky33对灰葡萄孢高度敏感。我们鉴定出超过1680个与1576个拟南芥基因相关的灰葡萄孢诱导的WRKY33结合位点。转录谱分析确定了接种后14小时的318个功能性直接靶基因。比较分析表明，WRKY33具有双重功能，在启动子背景依赖的方式下既可以作为阻遏物也可以作为激活剂发挥作用。我们证实了已知的参与激素信号传导和植物抗毒素生物合成的WRKY33靶标，但也揭示了脱落酸（ABA）在对灰葡萄孢2100抗性中的新的负作用。ABA生物合成基因NCED3和NCED5被鉴定为WRKY33介导的抗性所需的直接靶标。WRKY33功能丧失导致ABA水平升高，遗传研究证实WRKY33在NCED3/NCED5上游起作用以负调节ABA生物合成。这项研究首次详细阐述了特定植物转录因子在调节与植物免疫相关的转录网络中的全基因组贡献。

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